Determination of MDMA, MDEA and MDA in urine by high performance liquid chromatography with fluorescence detection

被引：32
|
作者：
da Costa, JL [1 ]
Chasin, AAD
机构：
[1] Univ Sao Paulo, Coll Pharmaceut Sci Toxicol, Instrumental Anal Lab, Criminalist Inst, Av Prof Lineu Prestes,580 B13B Toxicol, BR-05508900 Sao Paulo, Brazil
[2] Coll Oswaldo Cruz, Forens Toxicol Lab, Legal Med Inst, Sao Paulo, Brazil
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 811卷 / 01期
关键词：
drug of abuse; MDMA; MDEA;
D O I：
10.1016/j.jchromb.2004.03.076
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
This paper describes the development and validation of analytical methodology for the determination of the use of MDMA, MDEA and MDA in urine. After a simple liquid extraction, the analyses were carried out on a high performance liquid chromatography (HPLC) in an octadecyl column, with fluorescence detection. The mobile phase using a sodium dodecyl sulfate ion-pairing reagent allows good separation and efficiency. The method showed good linearity and precision. Recovery was between 85 and 102% and detection limits were 10, 15 and 20 ng/ml for MDA, MDMA and MDEA, respectively. No interfering substances were detected with fluorescence detection. (C) 2004 Elsevier B.V. All rights reserved.
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页码：41 / 45
页数：5
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