LINC00665 promotes Ovarian Cancer progression through regulating the miRNA-34a-5p/E2F3 axis

被引：16

作者：

Xu, Dan ^{[1
,3
]}

Song, Qingxia ^{[1
]}

Liu, Ying ^{[1
]}

Chen, Wansu ^{[1
]}

Lu, Lijuan ^{[1
]}

Xu, Min ^{[1
]}

Fang, Xiaohui ^{[4
]}

Zhao, Wenjie ^{[1
]}

Zhou, Huifang ^{[2
,3
]}

机构：

[1] Nanjing Univ Chinese Med, Dept Gynaecol, Suzhou TCM Hosp, Suzhou 215009, Peoples R China

[2] Nanjing Univ Chinese Med, Dept Gynaecol, Affiliated Hosp, Nanjing 210029, Peoples R China

[3] Nanjing Univ Chinese Med, Nanjing 210023, Peoples R China

[4] Nanjing Univ Chinese Med, Dept Clin Lab, Suzhou TCM Hosp, Suzhou 215009, Peoples R China

来源：

JOURNAL OF CANCER | 2021年 / 12卷 / 06期

关键词：

ovarian cancer; LINC00665; miRNA-34a-5p; E2F3;

D O I：

10.7150/jca.51457

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Objective: To clarify the role of LINC00665 in ovarian cancer (OC) progression and the possible mechanism. Methods: LINC00665 levels in OC tissues and cell lines were detected by qRT-PCR. The correlation between LINC00665 and clinicopathologic characteristics of OC patients was assessed. Biological functions of OC cell phenotypes influenced by LINC00665 were examined by CCK-8, colony formation and Transwell assay. Dual-luciferase reporter assay and RIP assay were conducted to verify the interaction between LINC00665 and its downstream target. Results: LINC00665 was upregulated in OC and linked to poor prognosis. Knockdown of LINC00665 blocked malignant proliferative, migratory and invasive functions of OC cells. By competitively binding miRNA-34a-5p, LINC00665 abolished the inhibitory effect of miR-34a-3p on its downstream gene E2F3, thus promoting OC progression. Conclusion: LINC00665/miRNA-34a-5p/E2F3 axis is involved in OC progression, providing novel insights into the clinical treatment of OC.

引用

页码：1755 / 1763

页数：9

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