Low KLOTHO level related to aging is associated with diminished ovarian reserve

Objective: To explore the relationship between KLOTHO expression and diminished ovarian reserve (DOR). Design: A case-control study. Setting: Reproductive medicine center. Patient(s): A total of 157 patients with DOR and 159 control women were recruited from the Centre of Reproductive Medicine, Peking University Third Hospital. Intervention(s): None. Main Outcome Measure(s): The granulosa cells were isolated from follicular fluid after oocyte retrieval, and the KLOTHO level of granulosa cell was measured using a modified quantitative polymerase chain reaction technique. The serum KLOTHO level was measured by solid-phase sandwich enzyme-linked immunosorbent assay. Result(s): In both granulosa cells and serum derived from women with DOR, KLOTHO expressions were significantly lower compared with normal ovarian reserve controls. Moreover, KLOTHO expression diminished with advancing age. Conclusion(s): Diminished KLOTHO expression was associated with DOR. Further longitudinal studies in a similar population accompanying disease progression and mechanism exploration are needed to substantiate the rules of KLOTHO in reproductive aging. ((c) 2020 by American Society for Reproductive Medicine.) El resumen esta disponible en Espanol al final del articulo.