High expression of the xylanase B gene from Clostridium stercorarium in tobacco cells

被引：13
|
作者：
Sun, JL
Kawazu, T
Kimura, T
Karita, S
Sakka, K
Ohmiya, K
机构：
[1] MIE UNIV,SCH BIORESOURCES,TSU,MIE 514,JAPAN
[2] MIE UNIV,CTR MOL BIOL & GENET,TSU,MIE 514,JAPAN
[3] NEW OJI INST FOREST TREE IMPROVEMENT,KAMEYAMA 51902,JAPAN
来源：
JOURNAL OF FERMENTATION AND BIOENGINEERING | 1997年 / 84卷 / 03期
关键词：
Clostridium stercorarium; tobacco suspension cell; xylanase;
D O I：
10.1016/S0922-338X(97)82057-0
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The xylanase gene (xynB) from Clostridium stercorarium was expressed in tobacco cells (BY2) under the control of the cauliflower mosaic virus (CaMV) 3SS promoter. The gene product, xylanase B (XynB), was proteolytically processed in the transformed cells (termed B1-11): a larger form of the protein was mainly found in the culture supernatant and a smaller one was found in the cells. The amount of XynB protein was estimated to be 5% of the total soluble proteins in B1-11 calli after 2 weeks of cultivation. XynB isolated from the transformed tobacco cells had the activity to hydrolyze the cell wall of barley straw.
引用
收藏
页码：219 / 223
页数：5
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