Smad3 activates the Sox9-dependent transcription on chromatin

被引:73
|
作者
Furumatsu, Takayuki [1 ,2 ]
Ozaki, Toshifumi [1 ]
Asahara, Hiroshi [2 ,3 ]
机构
[1] Okayama Univ, Grad Sch, Dept Orthopaed Surg, Okayama 7008558, Japan
[2] Scripps Res Inst, Dept Mol & Expt Med, La Jolla, CA 92037 USA
[3] Natl Ctr Child Hlth & Dev, Tokyo 1578535, Japan
基金
日本学术振兴会;
关键词
Chondrogenesis; Chromatin; p300; Smad3; Sox9; NUCLEOSOME ASSEMBLY PROTEIN-1; MESENCHYMAL STEM-CELLS; GENE-EXPRESSION; REGULATES CHONDROGENESIS; II COLLAGEN; SOX9; P300; DIFFERENTIATION; COACTIVATORS; KINASE;
D O I
10.1016/j.biocel.2008.10.032
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Transforming growth factor (TGF)-beta has an essential role for the Sry-type high-mobility-group box (Sox)-regulated chondrogenesis. Chondrogenic differentiation is also controlled by chromatin-mediated transcription. We have previously reported that TGF-beta-regulated Smad3 induces chondrogenesis through the activation of Sox9-dependent transcription. However, the cross-talk between TGF-beta signal and Sox9 on chromatin-mediated transcription has not been elucidated. In the present study, we investigated the activity of Smad3, Sox9, and coactivator p300 using an in vitro chromatin assembly model. Luciferase reporter assays revealed that Smad3 stimulated the Sox9-mediated transcription in a TGF-beta-dependent manner. Recombinant Sox9 associated with phosphorylated Smad3/4 and recognized the enhancer region of type 11 collagen gene. In vitro transcription and S1 nuclease assays showed that Smad3 and p300 cooperatively activated the Sox9-dependent transcription on chromatin template. The combination treatment of phosphorylated Smad3, Sox9, and p300 were necessary for the activation of chromatin-mediated transcription. These findings suggest that TGF-beta signal Smad3 plays a key role for chromatin remodeling to induce chondrogenesis via its association with Sox9. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码:1198 / 1204
页数:7
相关论文
共 50 条
  • [41] Genome-wide identification of Sox8-, and Sox9-dependent genes during early post-natal testis development in the mouse
    Chalmel, F.
    Lardenois, A.
    Georg, I.
    Barrionuevo, F.
    Demougin, P.
    Jegou, B.
    Scherer, G.
    Primig, M.
    ANDROLOGY, 2013, 1 (02) : 281 - 292
  • [42] TGF-β activates cyclin D1 expression through a Smad3/β-catenin-dependent mechanism in chondrocytes.
    Li, T.
    Chen, M.
    Wu, Q.
    Sheu, T.
    Drissi, H.
    Zuscik, M.
    Chen, D.
    O'Keefe, R. J.
    JOURNAL OF BONE AND MINERAL RESEARCH, 2006, 21 : S91 - S91
  • [43] Phenytoin activates SMAD3 phosphorylation and periostin expression in drug-induced gingival enlargement
    Kim, Shawna S.
    Nikoloudaki, Georgia
    Darling, Mark
    Rieder, Michael J.
    Hamilton, Douglas W.
    HISTOLOGY AND HISTOPATHOLOGY, 2018, 33 (12) : 1287 - 1298
  • [44] Smad3 induces chondrogenesis through the activation of SOX9 via CREB-binding protein/p300 recruitment
    Furumatsu, T
    Tsuda, M
    Taniguchi, N
    Tajima, Y
    Asahara, H
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (09) : 8343 - 8350
  • [45] Smad2 and Smad3 positively and negatively regulate TGFβ-dependent transcription through the forkhead DNA-Binding protein FAST2
    Labbe, E
    Silvestri, C
    Hoodless, PA
    Wrana, JL
    Attisano, L
    MOLECULAR CELL, 1998, 2 (01) : 109 - 120
  • [46] Smad3 Regulates Neuropilin 2 Transcription by Binding to its 5′ Untranslated Region
    Xie, Xiujie
    Urabe, Go
    Marcho, Lynn
    Williams, Corey
    Guo, Lian-Wang
    Kent, K. Craig
    JOURNAL OF THE AMERICAN HEART ASSOCIATION, 2020, 9 (08):
  • [47] Sox9 and p300 cooperatively regulate chromatin-mediated transcription
    Furumatsu, T
    Tsuda, M
    Yoshida, K
    Taniguchi, N
    Ito, T
    Hashimoto, M
    Ito, T
    Asahara, H
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2005, 280 (42) : 35203 - 35208
  • [48] Enhanced glomerular transfection by BMP7 gene nanocarriers inhibits CKD and promotes SOX9-dependent tubule regeneration
    Tai, Yifan
    Liu, Zhen
    Wang, Yanrong
    Zhang, Xinyan
    Li, Ruifang
    Yu, Jiangyue
    Chen, Yao
    Zhao, Lili
    Li, Jia
    Bai, Xueyuan
    Kong, Deling
    Midgley, Adam C.
    Nano Today, 2024, 59
  • [49] SMAD family member 3 (SMAD3) and SMAD4 repress HIF2-dependent iron-regulatory genes
    Ma, Xiaoya
    Das, Nupur K.
    Castillo, Cristina
    Gourani, Ayla
    Perekatt, Ansu O.
    Verzi, Michael P.
    Shah, Yatrik M.
    JOURNAL OF BIOLOGICAL CHEMISTRY, 2019, 294 (11) : 3974 - 3986
  • [50] The IFITM5 mutation in osteogenesis imperfecta type V is associated with an ERIC/SOX9-dependent osteoprogenitor differentiation defect
    Marom, Ronit
    Song, I. -Wen
    Busse, Emily C.
    Washington, Megan E.
    Berrier, Ava S.
    Rossi, Vittoria C.
    Ortinau, Laura
    Jeong, Youngjae
    Jiang, Ming-Ming
    Dawson, Brian C.
    Adeyeye, Mary
    Leynes, Carolina
    Lietman, Caressa D.
    Stroup, Bridget M.
    Batkovskyte, Dominyka
    Jain, Mahim
    Chen, Yuqing
    Cela, Racel
    Castellon, Alexis
    Tran, Alyssa A.
    Lorenzo, Isabel
    Meyers, D. Nicole
    Huang, Shixia
    Turner, Alicia
    Shenava, Vinitha
    Wallace, Maegen
    Orwoll, Eric
    Park, Dongsu
    Ambrose, Catherine G.
    Nagamani, Sandesh C. S.
    Heaney, Jason D.
    Lee, Brendan H.
    JOURNAL OF CLINICAL INVESTIGATION, 2024, 134 (15):