Methodology for intravital mitotic chromosome preparation from regenerated tissue derived from the tail tips of tadpoles

被引：0

作者：

Pustovalova, Eleonora ^{[1
]}

Fedorova, Anna ^{[1
]}

Shabanov, Dmytro ^{[1
]}

机构：

[1] Kharkov Natl Univ, Sch Biol, Dept Zool & Anim Ecol, Kharkiv, Ukraine

来源：

JOURNAL OF VERTEBRATE BIOLOGY | 2022年 / 71卷

关键词：

karyoanalysis; Pelophylax; polyploidy; regeneration; RANA-ESCULENTA; WATER FROGS; COMPLEX; NUMBER; DNA;

D O I：

10.25225/jvb.22010

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

We propose a modified and updated protocol to obtain mitotic chromosomes from the regenerated tissue of Pelophylax tadpole tail tips. Chromosomal preparations from regenerated tissue results in high-quality and dean slides suitable for further staining and study. Tadpoles remain alive, undergo minimum suffering, and can be grown to adulthood for further investigation. The method could be used for other groups of Anura and modified for other species with the ability to regenerate their tissues.

引用

页数：7

共 29 条

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