Calcium and cyclic nucleotides affect TNF-α-induced stem cell migration

被引：1
|
作者：
Kaplan, Emel [1 ,2 ]
Min, Jiang-Yong [2 ]
Ke, Qingen [2 ]
Chen, Yu [2 ]
Niethammer, Marc [3 ]
Rana, Jamal S. [2 ]
Malek, Sohail [2 ]
Verheugt, Freek W. A. [4 ]
Morgand, James P. [5 ]
机构：
[1] Radboud Univ Nijmegen, Dept Cardiol, Med Ctr, NL-6525 GA Nijmegen, Netherlands
[2] Harvard Univ, Sch Med, Beth Israel Deaconess Med Ctr, Boston, MA USA
[3] Harvard Univ, Sch Med, Brigham & Womens Hosp, Boston, MA USA
[4] Onze Lieve Vrouw Hosp, Amsterdam, Netherlands
[5] Tufts Univ, Caritas St Elizabeths Med Ctr, Boston, MA 02111 USA
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2009年 / 382卷 / 02期
关键词：
Stem cell migration; TNF-alpha; Calcium; cAMP; cGMP; Fura-2; fluorescence; KINASE; MOTILITY; ACTIVATION;
D O I：
10.1016/j.bbrc.2009.02.068
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The purpose of this Study was to study the effect of calcium, cyclic AMP (cAMP) and cyclic GMP (cGMP) on embryonic stem cell (ESC) motility during TNF-alpha-induced chemotaxis. ESCs were monitored using a chemotaxis chamber, with different concentrations of calcium or cAMP or cGMP added to the medium. Changes in intracellular calcium ([Ca2+](i)) were measured with the fluorescent dye fura-2/AM. We combined migratory parameters in a mathematical model and described it as 'mobility". After adding Calcium, a dose-dependant increase in cell speed was found. Cyclic AMP increased mobility as well as the [Ca2+](i). In contrast, adding dbcGMP resulted in a significant decrease in the mobility of the ESCs. During migration ESCs showed an increase in [Ca2+](i). Furthermore, TNF-alpha dramatically increased the movement as well as the directionality of ESCs. These results demonstrate that ESCs are highly motile and respond to different concentrations of calcium in a dose-related manner. (C) 2009 Elsevier Inc. All rights reserved.
引用
收藏
页码：241 / 246
页数：6
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