High-level production of soluble tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL) in high-density cultivation of recombinant Escherichia coli using a combined feeding strategy

被引：34
|
作者：
Shen, YL [1 ]
Zhang, Y [1 ]
Sun, AY [1 ]
Xia, XX [1 ]
Wei, DZ [1 ]
Yang, SL [1 ]
机构：
[1] E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2004年 / 26卷 / 12期
基金：
国家高技术研究发展计划(863计划);
关键词：
combined feeding strategy; Escherichia coli; fed-batch; high-cell-density cultivation; TRAIL;
D O I：
10.1023/B:BILE.0000030043.84226.ac
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Recombinant Escherichia coli strain C600/pBV-TRAIL (encoding for 114-281 amino acids of TRAIL's soluble fragment) produced a recombinant human tumor necrosis factor-related apoptosis-inducing ligand (Apo2L/TRAIL). Using a combined strategy of exponential feeding and pH-stat feeding, high concentrations of biomass (65 g dry wt l(-1)) and active soluble TRAIL (1.4 g l(-1)) were obtained within 30 h. The accumulation of acetate, which usually occurs during the process of high-density culture of Escherichia coli and especially in the induction stage of protein synthesis, was avoided.
引用
收藏
页码：981 / 984
页数：4
相关论文
共 38 条
  • [21] Preclinical studies to predict the disposition of Apo2L/tumor necrosis factor-related apoptosis-inducing ligand in humans: Characterization of in vivo efficacy, pharmacokinetics, and safety
    Kelley, SK
    Harris, LA
    Xie, D
    Deforge, L
    Totpal, K
    Bussiere, J
    Fox, JA
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2001, 299 (01): : 31 - 38
  • [22] Ligand-based targeting of apoptosis in cancer: The potential of recombinant human apoptosis ligand 2/tumor necrosis factor-related apoptosis-inducing ligand (rhApo2L/TRAIL)
    Ashkenazi, Avi
    Holland, Pamela
    Eckhardt, S. Gail
    JOURNAL OF CLINICAL ONCOLOGY, 2008, 26 (21) : 3621 - 3630
  • [23] Adriamycin sensitizes the adriamycin-resistant 8226/Dox40 human multiple myeloma cells to Apo2L/tumor necrosis factor-related apoptosis-inducing ligand-mediated (TRAIL) apoptosis
    Jazirehi, AR
    Ng, CP
    Gan, XH
    Schiller, G
    Bonavida, B
    CLINICAL CANCER RESEARCH, 2001, 7 (12) : 3874 - 3883
  • [24] Resistance to Apo2 Ligand (Apo2L)/Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand (TRAIL)-Mediated Apoptosis and Constitutive Expression of Apo2L/TRAIL in Human T-Cell Leukemia Virus Type 1-Infected T-Cell Lines (Retraction of vol 79, pg 1367, 2005)
    Matsuda, Takehiro
    Almasan, Alex
    Tomita, Mariko
    Uchihara, Jun-Nosuke
    Masuda, Masato
    Ohshiro, Kazuiku
    Takasu, Nobuyuki
    Yagita, Hideo
    Ohta, Takao
    Mori, Naoki
    JOURNAL OF VIROLOGY, 2011, 85 (03) : 1416 - 1416
  • [25] Apoptosis induction in prostate cancer cells and xenografts by combined treatment with Apo2 ligand/tumor necrosis factor-related apoptosis-inducing ligand and CPT-11
    Ray, S
    Almasan, A
    CANCER RESEARCH, 2003, 63 (15) : 4713 - 4723
  • [26] Intracellular regulation of tumor necrosis factor (TNF)-Related Apoptosis Inducing Ligand (TRAIL/Apo2L)-induced apoptosis in human multiple myeloma cells.
    Mitsiades, N
    Mitsiades, CS
    Poulaki, V
    Anderson, KC
    Treon, SP
    BLOOD, 2001, 98 (11) : 376A - 376A
  • [27] Vitamin E succinate is a potent novel antineoplastic agent with high selectivity and cooperativity with tumor necrosis factor-related apoptosis-inducing ligand (Apo2 ligand) in vivo
    Weber, T
    Lu, M
    Andera, L
    Lahm, H
    Gellert, N
    Fariss, MW
    Korinek, V
    Sattler, W
    Ucker, DS
    Terman, A
    Schröder, A
    Erl, W
    Brunk, UT
    Coffey, RJ
    Weber, C
    Neuzil, J
    CLINICAL CANCER RESEARCH, 2002, 8 (03) : 863 - 869
  • [28] Curcumin sensitizes prostate cancer cells to tumor necrosis factor-related apoptosis-inducing ligand/Apo2L by inhibiting nuclear factor-κB through suppression of IκBα phosphorylation
    Deeb, D
    Jiang, H
    Gao, XH
    Hafner, MS
    Wong, H
    Divine, G
    Chapman, RA
    Dulchavsky, SA
    Gautam, SC
    MOLECULAR CANCER THERAPEUTICS, 2004, 3 (07) : 803 - 812
  • [29] Osteoprotegerin is a soluble decoy receptor for tumor necrosis factor-related apoptosis-inducing ligand/Apo2 ligand and can function as a paracrine survival factor for human myeloma cells
    Shipman, CM
    Croucher, PI
    CANCER RESEARCH, 2003, 63 (05) : 912 - 916
  • [30] Enhanced production of soluble tumor necrosis factor-related apoptosis-inducing ligand in Escherichia coli using a novel self-cleavable tag system Fh8-ΔI-CM
    Zhang, Min
    Wang, Zhanqing
    Chi, Lili
    Sun, Jing
    Shen, Yaling
    PROTEIN EXPRESSION AND PURIFICATION, 2018, 148 : 16 - 23
1234