The ryanodine receptor Subtype 3 (RYR3) is expressed ubiquitously but its physiological function varies from cell to cell. Here, we investigated the role of a dominant negative RYR3 isoforni in Ca2+ signalling in native smooth muscle cells. We used intranuclear injection of antisense oligonucleotides to specifically inhibit endogenous RYR3 isoform expression. In mouse duodenum myocytes expressing RYR2 subtype and both spliced and non-spliced RYR3 isoforms, RYR2 and non-spliced RYR3 were activated by caffeine whereas the spliced RYR3 was not. Only RYR2 was responsible for the Ca2+-induced Ca2+ release mechanism that amplified Ca2+ influx- or inositol 1,4,5-trisphosphate-induced Ca2+ signals. However, the spliced RYR3 negatively regulated RYR2 leading to the decrease of amplitude and upstroke velocity of Ca2+ signals. Immunostaining in injected cells showed that the spliced RYR3 was principally expressed near the plasma membrane whilst the non-spliced isoform was revealed around the nucleus. This study shows for the first time that the short isoform of RYR3 controls Ca2+ release through RYR2 in native smooth muscle cells. (c) 2006 Elsevier Ltd. All rights reserved.
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Li, Hong-Xia .
Kong, Fan-Juan
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Kong, Fan-Juan
Bai, Shu-Zhi
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Bai, Shu-Zhi
He, Wen
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
He, Wen
Xing, Wen-Jing
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Xing, Wen-Jing
Xi, Yu-Hui
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Xi, Yu-Hui
Li, Guang-Wei
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Li, Guang-Wei
Guo, Jin
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Guo, Jin
Li, Hong-Zhu
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Li, Hong-Zhu
Wu, Ling-Yun
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R China
Lakehead Univ, Dept Biol, Thunder Bay, ON P7B 5E1, CanadaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Wu, Ling-Yun
Wang, Rui
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R China
Lakehead Univ, Dept Biol, Thunder Bay, ON P7B 5E1, CanadaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Wang, Rui
Yang, Guang-Dong
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Lakehead Univ, Dept Biol, Thunder Bay, ON P7B 5E1, CanadaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Yang, Guang-Dong
Tian, Ye
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Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Tian, Ye
Xu, Chang-Qing
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Biopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
Harbin Med Coll, Dept Pathophysiol, Harbin 150086, Peoples R ChinaBiopharmaceut Key Lab Heilongjiang Prov, Harbin 150086, Peoples R China
机构:
Sichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China
Sichuan Univ, West China Hosp, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China
Luo, Jian
Liu, Yuan-hua
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Zhengzhou Univ, Affiliated Hosp 1, Dept Resp Dis, Zhengzhou 450052, Peoples R ChinaSichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China
Liu, Yuan-hua
Luo, Wei
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Chongqing Med Univ, Affiliated Hosp 1, Dept Resp Dis, Chongqing 400016, Peoples R ChinaSichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China
Luo, Wei
Luo, Zhu
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Sichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China
Sichuan Univ, West China Hosp, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China
Luo, Zhu
Liu, Chun-tao
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Sichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China
Sichuan Univ, West China Hosp, Chengdu 610041, Peoples R ChinaSichuan Univ, West China Sch Med, Dept Resp Dis, Chengdu 610041, Peoples R China