Visualisation of polymer distribution in polyelectrolyte microcapsules by light scattering and multiple fluorescence labelling

被引：0
|
作者：
Podskocova, J. [1 ]
Chorvat, D., Jr. [1 ]
Kollarikova, G. [1 ]
Lacik, I [1 ]
机构：
[1] Comenius Univ, Fac Math Phys & Informat, Bratislava, Slovakia
来源：
SARATOV FALL MEETING 2005: OPTICAL TECHNOLOGIES IN BIOPHYSICS AND MEDICINE VII | 2006年 / 6163卷
关键词：
polyelectrolyte microcapsules; multiple fluorescence labelling; linear unmixing;
D O I：
10.1117/12.697290
中图分类号：
Q6 [生物物理学];
学科分类号：
071011 ;
摘要：
In this contribution we show application of multispectral confocal laser scanning microscopy for visualisation and characterisation of polyelectrolyte microcapsules. Fluorescent labels of different free charges, specifically bound by electrostatic interactions to the polymer chains in polyelectrolyte microcapsules, were used for questioning the spatial distribution of polymer components of the microcapsules. To separate the overlapping emission spectra of the fluorescent labels spectral imaging and linear unmixing approaches was used.
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页数：8
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