ADHERENCE ASSAYS AND SLIME PRODUCTION OF VIBRIO ALGINOLYTICUS AND VIBRIO PARAHAEMOLYTICUS

被引：0

作者：

Abdallah, Fethi Ben ^{[1
,2
]}

Chaieb, Kamel ^{[1
]}

Zmantar, Tarek ^{[1
]}

Kallel, Hela ^{[2
]}

Bakhrouf, Amina ^{[1
]}

机构：

[1] Fac Pharm, Lab Anal Traitement & Valorisat Polluants Environ, Monastir 5000, Tunisia

[2] Inst Pasteur Tunis, Unite Fermentat & Dev Vaccins Virol, Tunis 1002, Tunisia

来源：

BRAZILIAN JOURNAL OF MICROBIOLOGY | 2009年 / 40卷 / 02期

关键词：

Vibrio; slime production; adherence; abiotic surface; Vero cells; COAGULASE-NEGATIVE STAPHYLOCOCCI; BIOFILM FORMATION; SURFACES; CELLS; ATTACHMENT; VIRULENCE; IDENTIFICATION; BACTERIA; ADHESION; STRAINS;

D O I：

10.1590/S1517-83822009000200033

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

In this study we investigated the phenotypic slime production of Vibrio alginolyticus and Vibrio parahaemolyticus strains, food-borne pathogens, using a Congo red agar plate assay. Furthermore, we studied their ability to adhere to abiotic surfaces and Vero cells line. Our results showed that only V. alginolyticus ATCC 17749 was a slime-producer developing almost black colonies on Congo red agar plate. Adherence to glace tube showed that all V. alginolyticus strains were more adherent than V. parahaemolyticus. Only V. alginolyticus ATCC 17749 was found to be able to form biofilm on polystyrene microplate wells (OD570 = 0.532). Adherence to Vero cells showed that all tested strains were non adherent after 30 min, however after 60 min all the studied strains become adherent. The percentage of adherence ranged from 1.23% to 4.66%.

引用

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页码：394 / 398

页数：5

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