In Vivo CRISPR-Cas Genome Editing with No Detectable Off-Target Mutations

被引:0
|
作者
Bobbin, Maggie L. [1 ,2 ]
Akcakaya, Pinar [3 ]
Guo, Jimmy [1 ,2 ]
Fellows, Mick [4 ]
Porritt, Michelle [3 ]
Firth, Mike [3 ]
Carreras, Alba [3 ]
Baccega, Tania [3 ]
Tsai, Shengdar [1 ,2 ]
Nguyen, Nhu T. [1 ,2 ]
Seeliger, Frank [5 ]
Bjursell, Mikael [3 ]
Nitsch, Roberto [4 ]
Lopez, Jose M. [1 ,2 ]
Clement, Kendell [1 ,2 ]
Garcia, Sara P. [1 ,2 ]
Hsu, Jonathan Y. [1 ,2 ]
Pinello, Luca [1 ,2 ]
Aryee, Martin [1 ,2 ]
Mayr, Lorenz M. [3 ]
Bohlooly-Y, Mohammad [3 ]
Maresca, Marcello [3 ]
Joung, J. Keith [1 ,2 ]
机构
[1] MGH, Pathol, Boston, MA USA
[2] Harvard Med Sch, Pathol, Boston, MA USA
[3] AstraZeneca, IMED Biotech Unit, Discovery Biol, Discovery Sci, Gothenburg, Sweden
[4] AstraZeneca, IMED Biotech Unit, New Drug Modal Drug Safety & Metab, Cambridge, England
[5] AstraZeneca, IMED Biotech Unit, Pathol Sci Drug Safety & Metab, Gothenburg, Sweden
关键词
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
42
引用
下载
收藏
页码:22 / 23
页数:2
相关论文
共 50 条
  • [31] GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
    Tsai, Shengdar Q.
    Zheng, Zongli
    Nguyen, Nhu T.
    Liebers, Matthew
    Topkar, Ved V.
    Thapar, Vishal
    Wyvekens, Nicolas
    Khayter, Cyd
    Iafrate, A. John
    Le, Long P.
    Aryee, Martin J.
    Joung, J. Keith
    NATURE BIOTECHNOLOGY, 2015, 33 (02) : 187 - 197
  • [32] GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases
    Shengdar Q Tsai
    Zongli Zheng
    Nhu T Nguyen
    Matthew Liebers
    Ved V Topkar
    Vishal Thapar
    Nicolas Wyvekens
    Cyd Khayter
    A John Iafrate
    Long P Le
    Martin J Aryee
    J Keith Joung
    Nature Biotechnology, 2015, 33 : 187 - 197
  • [33] A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex
    Marius Rutkauskas
    Inga Songailiene
    Patrick Irmisch
    Felix E. Kemmerich
    Tomas Sinkunas
    Virginijus Siksnys
    Ralf Seidel
    Nature Communications, 13
  • [34] A quantitative model for the dynamics of target recognition and off-target rejection by the CRISPR-Cas Cascade complex
    Rutkauskas, Marius
    Songailiene, Inga
    Irmisch, Patrick
    Kemmerich, Felix E.
    Sinkunas, Tomas
    Siksnys, Virginijus
    Seidel, Ralf
    NATURE COMMUNICATIONS, 2022, 13 (01)
  • [35] Heritable multiplex gene editing via CRISPR/Cas9 exhibits no detectable genome-wide off-target effects in sheep.
    Wang, X.
    Chen, Y.
    JOURNAL OF ANIMAL SCIENCE, 2016, 94 : 177 - 177
  • [36] Delivery of CRISPR-Cas tools for in vivo genome editing therapy: Trends and challenges
    Taha, Eman A.
    Lee, Joseph
    Hotta, Akitsu
    JOURNAL OF CONTROLLED RELEASE, 2022, 342 : 345 - 361
  • [37] On- and off-target effects of paired CRISPR-Cas nickase in primary human cells
    Klermund, Julia
    Rhiel, Manuel
    Kocher, Thomas
    Chmielewski, Kay Ole
    Bischof, Johannes
    Andrieux, Geoffroy
    el Gaz, Melina
    Hainzl, Stefan
    Boerries, Melanie
    Cornu, Tatjana I.
    Koller, Ulrich
    Cathomen, Toni
    MOLECULAR THERAPY, 2024, 32 (05)
  • [38] High-Fidelity CRISPR-Cas9 Nucleases with No Detectable Genome-Wide Off-Target Effects
    Kleinstiver, Benjamin P.
    Pattanayak, Vikram
    Prew, Michelle S.
    Tsai, Shengdar Q.
    Nguyen, Nhu T.
    Joung, J. Keith
    MOLECULAR THERAPY, 2016, 24 : S288 - S288
  • [39] High-fidelity CRISPR–Cas9 nucleases with no detectable genome-wide off-target effects
    Benjamin P. Kleinstiver
    Vikram Pattanayak
    Michelle S. Prew
    Shengdar Q. Tsai
    Nhu T. Nguyen
    Zongli Zheng
    J. Keith Joung
    Nature, 2016, 529 : 490 - 495
  • [40] High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects
    Kleinstiver, Benjamin P.
    Pattanayak, Vikram
    Prew, Michelle S.
    Tsai, Shengdar Q.
    Nguyen, Nhu T.
    Zheng, Zongli
    Joung, J. Keith
    NATURE, 2016, 529 (7587) : 490 - +