Celecoxib combined with salirasib strongly inhibits pancreatic cancer cells in 2D and 3D cultures

被引：5

作者：

Li, Dongli ^{[1
,2
]}

Ma, Yuran ^{[1
]}

Liu, Wenfeng ^{[1
]}

Ren, Xiang ^{[1
]}

Chen, Min ^{[1
]}

Xu, Xuetao ^{[1
]}

Sheng, Zhaojun ^{[1
]}

Zhang, Kun ^{[1
]}

Zhou, Renping ^{[3
]}

Goodin, Susan ^{[4
]}

Zheng, Xi ^{[1
,3
]}

机构：

[1] Wuyi Univ, Sch Biotechnol & Hlth Sci, Jiangmen 529020, Peoples R China

[2] Int Healthcare Innovat Inst Jiangmen, Jiangmen City 529020, Guangdong, Peoples R China

[3] Rutgers State Univ, Ernest Mario Sch Pharm, Dept Biol Chem, Piscataway, NJ 08854 USA

[4] Rutgers Canc Inst New Jersey, New Brunswick, NJ 08903 USA

来源：

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES | 2020年 / 17卷 / 12期

关键词：

Pancreatic cancer; 3D cultures; celecoxib; salirasib; NF-kappa B; NF-KAPPA-B; GROWTH; APOPTOSIS; EXPRESSION; PATHWAY; CYCLOOXYGENASE-2; ANGIOGENESIS; COMBINATION; SUPPRESSION; MUTATION;

D O I：

10.7150/ijms.47546

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background/Aim: Pancreatic adenocarcinoma is a highly malignant tumor. Synergistic combinations of anticancer agents for the effective treatment of pancreatic cancer patients are urgently needed. Here, we investigated the combined effect of celecoxib (CEL) and salirasib (SAL) on pancreatic cancer cells. Methods: Cell viability and apoptosis were measured by the trypan blue assay, three-dimensional cultures, propidium iodide staining, and caspase-3 assay. NF-kappa B activation and the protein levels of Akt, pAkt, and Bcl-2 were determined by the luciferase reporter assay and western blot. Results: Co-treatment with CEL and SAL had stronger effects on decreasing cell viability and inducing apoptosis in Panc-1 cells as compared with each agent individually. This combination strongly inhibited NF-kappa B activity and reduced pAkt and Bcl-2 levels in Panc-1 cells. Conclusion: SAL in combination with CEL may represent a new approach for effective inhibition of pancreatic cancer.

引用

页码：1795 / 1802

页数：8

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