Single-molecule fluorescence studies of protein folding and conformational dynamics

被引:412
|
作者
Michalet, Xavier [1 ]
Weiss, Shimon [1 ]
Jager, Marcus [1 ]
机构
[1] Univ Calif Los Angeles, Dept Chem & Biochem, Los Angeles, CA 90095 USA
关键词
D O I
10.1021/cr0404343
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
A review of single-molecule methods in chemistry is made to provide a rapid description of the main technical approaches. Topics discussed include fluorescence spectroscopy as a tool for molecular conformation and fluctuation measurements, single-molecule data acquisition and analysis methods, data analysis methods, single-molecule fluorescence studies of protein folding and conformations at equilibrium, and single-molecule protein folding under nonequilibrium conditions.
引用
收藏
页码:1785 / 1813
页数:29
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