Cloning of bacterial PCB-degrading gene into the plants

The target of this work was cloning of bacterial degrading gene. bphC to increase biodegradation potencial of polychlorinated biphenyls (PCB) by the plants. For this purpose the gene bphC encoding the enzyme 2,3-dihydroxybiphenyl-1,2-dioxygenase from bacteria P testosteroni B-356 was chosen to be cloned with the detection marker gene GFP. Because of the difficulties with the detection of expression of GFP in plant tissue, also other constructs were prepared. These contain luciferase gene next to the bphC gene, GUS gene and/or six His. The presence of bphC/GFP DNA and RNA was proved by PCR. Immunochemical analysis confirmed the presence of BphC/GFP fusion in several transformants. The constructs with bphC/LUC, bphC/GUS and bphC/His were transformed into the X tabacum via agrobacterial infection.