Creation of targeted inversion mutations in plants using an RNA-guided endonuclease

被引：19
|
作者：
Zhang, Congsheng [1 ,2 ]
Liu, Changlin [1 ]
Weng, Jianfeng [1 ]
Cheng, Beijiu [2 ]
Liu, Fang [1 ]
Li, Xinhai [1 ]
Xie, Chuanxiao [1 ]
机构：
[1] Chinese Acad Agr Sci, Inst Crop Sci, Natl Key Facil Crop Gene Resources & Genet Improv, Beijing 100081, Peoples R China
[2] Anhui Agr Univ, Hefei 230036, Peoples R China
来源：
CROP JOURNAL | 2017年 / 5卷 / 01期
基金：
中国国家自然科学基金;
关键词：
RNA-guided endonuclease; CRISPR/Cas9; Targeted inversion; Genetic improvement; ZINC-FINGER NUCLEASES; CRISPR-CAS SYSTEM; ARABIDOPSIS-THALIANA; GENOME; GENE; EVOLUTION; RICE; REARRANGEMENTS; DUPLICATION; MECHANISMS;
D O I：
10.1016/j.cj.2016.08.001
中图分类号：
S3 [农学（农艺学）];
学科分类号：
0901 ;
摘要：
Inversions are DNA rearrangements that are essential for plant gene evolution and adaptation to environmental changes. We demonstrate the creation of targeted inversions and previously reported targeted deletion mutations via delivery of a pair of RNA-guided endonucleases (RGENs) of CRISPR/Cas9. The efficiencies of the targeted inversions were 2.6% and 2.2% in the Arabidopsis FLOWERING TIME (AtFT) and TERMINAL FLOWER 1 (AtTFL1) loci, respectively. Thus, we successfully established an approach that can potentially be used to introduce targeted DNA inversions of interest for functional studies and crop improvement. (C) 2016 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
引用
收藏
页码：83 / 88
页数：6
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