Cytotoxic, Anti-Proliferative and Apoptosis Activity of L-Amino Acid Oxidase from Malaysian Cryptelytrops purpureomaculatus (CP-LAAO) Venom on Human Colon Cancer Cells

被引：17
|
作者：
Abidin, Syafiq Asnawi Zainal [1 ]
Rajadurai, Pathmanathan [1 ]
Chowdhury, Md. Ezharul Hoque [1 ]
Othman, Iekhsan [1 ]
Naidu, Rakesh [1 ]
机构：
[1] Monash Univ Malaysia, Jeffrey Cheah Sch Med & Hlth Sci, Jalan Lagoon Selatan, Bandar Sunway 47500, Selangor Darul, Malaysia
来源：
MOLECULES | 2018年 / 23卷 / 06期
关键词：
L-amino acid oxidase; Cryptelytrops purpureomaculatus; cytotoxicity; apoptosis; human colon cancer cells; ACUTUS SNAKE-VENOM; STRUCTURAL-CHARACTERIZATION; CALLOSELASMA-RHODOSTOMA; PURIFICATION; SUBSTRATE;
D O I：
10.3390/molecules23061388
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The aim of this study is to investigate the potential anti-cancer activity of L-amino acid oxidase (CP-LAAO) purified from the venom of Cryptelytrops purpureomaculatus on SW480 and SW620 human colon cancer cells. Mass spectrometry guided purification was able to identify and purify CP-LAAO. Amino acid variations identified from the partial protein sequence of CP-LAAO may suggest novel variants of these proteins. The activity of the purified CP-LAAO was confirmed with o-phenyldiamine (OPD)-based spectrophotometric assay. CP-LAAO demonstrated time-and dose-dependent cytotoxic activity and the EC50 value was determined at 13 mu g/mL for both SW480 and SW620 cells. Significant increase of caspase-3 activity, reduction of Bcl-2 levels, as well as morphological changes consistent with apoptosis were demonstrated by CP-LAAO. Overall, these data provide evidence on the potential anti-cancer activity of CP-LAAO from the venom of Malaysian C. purpureomaculatus for therapeutic intervention of human colon cancer.
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页数：16
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