Metagenomic approach reveals the fate of antibiotic resistance genes in a temperature-raising anaerobic digester treating municipal sewage sludge

被引:49
|
作者
Xu, Rui [1 ,2 ]
Zhang, Yanru [3 ,4 ]
Xiong, Weiping [3 ,4 ]
Sun, Weimin [1 ,2 ]
Fan, Qiao [5 ]
Yang, Zhaohui [3 ,4 ]
机构
[1] Guangdong Acad Sci, Guangdong Inst Ecoenvironm Sci & Technol, Guangdong Key Lab Integrated Agroenvironm Pollut, Guangzhou 510650, Peoples R China
[2] Natl Reg Joint Engn Res Ctr Soil Pollut Control &, Guangzhou 510650, Peoples R China
[3] Hunan Univ, Coll Environm Sci & Engn, Changsha 410082, Hunan, Peoples R China
[4] Hunan Univ, Key Lab Environm Biol & Pollut Control, Minist Educ, Changsha 410082, Hunan, Peoples R China
[5] Hunan Res Acad Environm Sci, Changsha 410082, Hunan, Peoples R China
基金
中国国家自然科学基金; 中国博士后科学基金;
关键词
Municipal sewage sludge; Antibiotic resistance genes; Thermophilic anaerobic digestion; Mesophilic anaerobic digestion; Metagenomic sequencing; MICROBIAL COMMUNITY; TETRACYCLINE RESISTANCE; CO-DIGESTION; MECHANISMS; BIOGAS; ACCUMULATION; INTEGRASE; RESISTOME; BACTERIA; REACTORS;
D O I
10.1016/j.jclepro.2020.123504
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The fate of diverse antibiotic resistance genes (ARGs) in anaerobic digestion (AD) system under different temperature remains largely unknow. This study employed the shotgun metagenomic sequencing and 16S rRNA amplicon sequencing approaches and investigated the shifts of composition and abundance of more than 1360 ARGs, as well as microbial community, in a temperature-raising AD system (from 35 degrees C to 55 degrees C) treating municipal sewage sludge. Results suggested that the six ARGs, inlcuding macB, tetA(58), bcrA, evgS, mtrA, and oleC, were predominated in digested sludge. More than 407ARG subtypes (30%) could be largely reduced under high temperature condition. Thermophilic AD (55 degrees C) provided a better removal for tetracycline, macrolide, penam, fluoroquinolone, acridine dye, and peptide resistance genes when compared to mesophilic AD (35 degrees C). The removal rates of these genes reached 29.7%-32.3%. In addition, at the end of thermophilic digestion process, the relative abundance of Firmicutes increased (from 18.22% to 74.89%), while Proteobacteria and Chloroflexi both decreased, from 36.39% to 6.80% and 20.29%-2.64%. This study further proposed the underlying mechanisms of effective elimination of ARGs under high temperature by: (1) the reduction of genes encoding antibiotic efflux pump, (2) the promotion of sludge biomass degradation rate, (3) the loss of microbial community diversity in AD systems, and (4) the inactivation of multidrug-resistant bacteria. The significance of current study is in characterizing the fate of ARGs across AD process, which allows to correlate diverse genetic properties with digesters' operational condition. Results provided an insight into the manipulation of AD technology (e.g. temperature) to efficiently remove ARGs from municipal sewage sludge. (c) 2020 Elsevier Ltd. All rights reserved.
引用
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页数:10
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