Bioorthogonal labelling of 3-nitrotyrosine in peptides and proteins through diazotisation mediated azidation

被引：2
|
作者：
Ng, John Y.
Wong, Jason W. H. [1 ]
机构：
[1] UNSW Australia, Lowy Canc Res Ctr, Sydney, NSW 2052, Australia
来源：
ORGANIC & BIOMOLECULAR CHEMISTRY | 2015年 / 13卷 / 02期
基金：
澳大利亚研究理事会;
关键词：
MASS-SPECTROMETRIC QUANTIFICATION; TYROSINE NITRATION; TERMINAL ALKYNES; NITRIC-OXIDE; HUMAN PLASMA; NITROTYROSINE; 3-AMINOTYROSINE; PEROXYNITRITE; DISEASE; TISSUES;
D O I：
10.1039/c4ob02133a
中图分类号：
O62 [有机化学];
学科分类号：
070303 ; 081704 ;
摘要：
A new chemical derivatisation method to functionalise peptide and protein bound 3-nitrotyrosine residues is described. By transforming 3-nitrotyrosine to 3-azidotyrosine, the method enables 'click chemistry'-based labelling of the 3-nitrotyrosine residues and provides new opportunities for the study of this post-translational modification in biological samples.
引用
收藏
页码：374 / 378
页数：5
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