Chiral packed column subcritical fluid chromatography on polysaccharide and macrocyclic antibiotic chiral stationary phases.

被引:108
|
作者
Medvedovici, A
Sandra, P
Toribio, L
David, F
机构
[1] STATE UNIV GHENT,DEPT ORGAN CHEM,ORGAN SYNTH LAB,B-9000 GHENT,BELGIUM
[2] RES INST CHROMATOG,B-8500 KORTRIJK,BELGIUM
关键词
chiral stationary phases; SFC; enantiomer separation; subcritical fluid chromatography; polysaccharide chiral stationary phases; macrocyclic antibiotic chiral stationary phases;
D O I
10.1016/S0021-9673(97)00585-2
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
The polysaccharide chiral stationary phases (CSPs) Chiralcel OD and Chiralpak AD, and the macrocyclic antibiotic CSPs Chirobiotic V and Chirobiotic T were evaluated in packed column subcritical fluid chromatography (pSFC) for the separation of different types of racemic compounds (beta-blockers, beta-agonists, benzodiazipines, non-steroidal anti-inflammatory drugs, barbiturates, free and derivatized amino acids, etc.). The same conditions and program could be applied to check the applicability and enantioselectivity of one of the CSPs for a given racemic mixture. The conditions are: temperature 30 degrees C, pressure 200 bar, flow-rate 2 ml/min, carbon dioxide, modifier methanol containing 0.1% trifluoroacetic acid (TFAA) and 0.1% triethylamine (TEA) with a gradient from 5% (5 min) to 30% at 5%/min. A resolution of 0.4 under those conditions generally indicates that baseline separation can be realized on the CSP by fine-tuning the different parameters of the SFC separation. The best CSP for pSFC proved to be Chiralpak AD which provided separation for 70% of the 44 substances tested, followed by Chiralcel OD (66%), Chirobiotic T (50%) and Chirobiotic V (48%). For comparison, the enantioselectivity in pSFC of the brush type CSPs Chirex 3022 with pi-donor characteristics and Chirex 3005 with pi-acceptor characteristics was also evaluated. As expected, these phases perform poorly under SFC conditions, on Chirex 3022 (34%) and on Chirex 3005 (20%). (C) 1997 Elsevier Science B.V.
引用
收藏
页码:159 / 171
页数:13
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