A new active antimicrobial peptide from PD-L4, a type 1 ribosome inactivating protein of Phytolacca dioica L.: A new function of RIPs for plant defence?

被引：23

作者：

Pizzo, Elio ^{[1
]}

Zanfardino, Anna ^{[1
]}

Di Giuseppe, Antonella M. A. ^{[2
]}

Bosso, Andrea ^{[1
]}

Landi, Nicola ^{[2
]}

Ragucci, Sara ^{[2
]}

Varcamonti, Mario ^{[1
]}

Notomista, Eugenio ^{[1
]}

Di Maro, Antimo ^{[2
]}

机构：

[1] Univ Naples Federico II, Dept Biol, I-80126 Naples, Italy

[2] Univ Naples 2, Dept Environm Biol & Pharmaceut Sci & Technol, I-81100 Caserta, Italy

来源：

FEBS LETTERS | 2015年 / 589卷 / 19期

关键词：

Antimicrobial peptide; Cationic antimicrobial peptide; Ribosome inactivating protein; Phytolacca dioica; GLYCOSIDASE ACTIVITY; EXPRESSION; RESISTANCE; MECHANISM; LYSOZYME; BACTERIA; TOBACCO; LEAVES; RICIN; SEEDS;

D O I：

10.1016/j.febslet.2015.08.018

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We investigated the antimicrobial activity of PD-L4, a type 1 RIP from Phytolacca dioica. We found that this protein is active on different bacterial strains both in a native and denatured/alkylated form and that this biological activity is related to a cryptic peptide, named PDL4(40-65), identified by chemical fragmentation. This peptide showed the same antimicrobial activity of full-length protein and possessed, similarly to several antimicrobial peptides, an immunomodulatory effect on human cells. It assumes an alpha-helical conformation when interact with mimic membrane agents as TFE and likely bacterial membranes are a target of this peptide. To date PDL4(40-65) is the first antimicrobial peptide identified in a type 1 RIP. (C) 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2812 / 2818

页数：7

共 24 条

[21] Molecular cloning and expression analysis on a new putative antimicrobial peptide gene encoding a defensin like protein from Allium cepa L.
Peng, Wu
RESEARCH JOURNAL OF BIOTECHNOLOGY, 2016, 11 (10): : 69 - 75
[22] Presence of polymerized and free forms of the non-toxic type 2 ribosome-inactivating protein ebulin and a structurally related new homodimeric lectin in fruits of Sambucus ebulus L.
Lucía Citores
Fernando M. de Benito
Rosario Iglesias
J. Miguel Ferreras
Pablo Argüeso
Pilar Jiménez
Enrique Méndez
Tomás Girbés
Planta, 1998, 204 : 310 - 317
[23] Presence of polymerized and free forms of the non-toxic type 2 ribosome-inactivating protein ebulin and a structurally related new homodimeric lectin in fruits of Sambucus ebulus L.
Citores, L
de Benito, FM
Iglesias, R
Ferreras, JM
Argüeso, P
Jiménez, P
Méndez, E
Girbés, T
PLANTA, 1998, 204 (03) : 310 - 317
[24] Structures of new acylated oleanene-type triterpene oligoglycosides, theasaponins E1 and E2, from the seeds of tea plant, Camellia sinensis (L.) O.!Kuntze
Kitagawa, I
Hori, K
Motozawa, T
Murakami, T
Yoshikawa, M
CHEMICAL & PHARMACEUTICAL BULLETIN, 1998, 46 (12) : 1901 - 1906

← 1 2 3 →