NMR Study of Poly(γ-Glutamic Acid) and Partially Benzylated Poly(γ-Glutamic Acid): Nanoparticles in Solution

被引：2
|
作者：
Nagy, Z. [1 ]
Novak, L. [1 ]
Kozma, C. [1 ]
Berka, M. [1 ]
Banyai, I. [1 ]
机构：
[1] Univ Debrecen, Fac Sci, Dept Colloid & Environm Chem, H-4032 Egyetem Tl, Hungary
来源：
COLLOIDS FOR NANO- AND BIOTECHNOLOGY | 2008年 / 135卷
关键词：
Aggregation; Amphipatic polymers; NMR diffusiometry; Poly(gamma-glutamic acid); Self-assembly;
D O I：
10.1007/2882_2008_112
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Poly(gamma-glutamic acid) (gamma-PGA) and its partially benzylamidated (hydrophobized) derivatives were prepared and studied in solution by means of dynamic light scattering (DLS), I D, 2D and PGSE NMR in order to determine the structure, size and aggregation in solution. By pH potentiometric titrations in aqueous solution the apparent pK approximate to 4 of gamma-PGA was determined. The measured diffusion coefficients as a function of pH showed the swelling of gamma-PGA by deprotonation of carboxyls. DLS measurements showed a certain degree of aggregation even in dilute solution. The degree of aggregation is increased in the presence H2PO4- and H2PO42- buffer anions. Benzylamidation of 30-50% of caboxylates resulted in folded structure of the BzPGA, probably spherical like nanoparticles, verified by NMR diffusiometry and 2D NOE spectroscopy.
引用
收藏
页码：200 / 208
页数：9
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