A simple method for determining Aphelenchoides besseyi infestation level of Oryza sativa seeds

被引：0

作者：

Hoshino, S ^{[1
]}

Togashi, K

机构：

[1] Hiroshima Univ, Fac Integrated Arts & Sci, Higashihiroshima, Hiroshima 7398521, Japan

[2] Hiroshima Prefectural Agr Res Ctr, Higashihiroshima, Hiroshima 7390151, Japan

来源：

JOURNAL OF NEMATOLOGY | 1999年 / 31卷 / 04期

关键词：

Aphelenchoides besseyi; assay; extraction; methodology; nematode; nematode load; Oryza sativa; rice; white tip disease;

D O I：

暂无

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

A simple extraction method was developed for obtaining the white tip nematode, Aphelenchoides besseyi Christie, from single seeds of rice, Oryza sativa. The method was as follows: Individual rice seeds were split longitudinally and then transferred into single pipet tips. Tips containing a split seed were then singly placed upright in glass vials dth water to extract the nematodes. This method was more efficient than the Baermann funnel technique and allowed nearly 100% recovery of living A. besseyi from single rice seeds within 4 hours.

引用

页码：641 / 643

页数：3

共 30 条

[21] A CONVENIENT METHOD FOR DETERMINING THE EFFECT OF ABSCISIC-ACID ON THE STOMATAL APERTURE OF RICE (ORYZA-SATIVA L) BY FEEDING SOLUTION TO THE LEAF TIP
KOBATA, T
HARA, S
JAPANESE JOURNAL OF CROP SCIENCE, 1994, 63 (04) : 638 - 642
[22] A SIMPLE METHOD OF DETERMINING 4-HYDROXYISOVALERIC ACID AND ITS LEVEL IN A PATIENT WITH ISOVALERIC ACIDEMIA
SHIGEMATSU, Y
KIKAWA, Y
SUDO, M
KIKUCHI, K
OHTA, S
OKAMOTO, M
CLINICA CHIMICA ACTA, 1984, 138 (03) : 333 - 336
[23] The validation of a simple LC/MS/MS method for determining the level of mevalonic acid in human plasma
Kindt, Erick
Szekely-Klepser, Gabriella
Fountain, Scott T.
BIOMEDICAL CHROMATOGRAPHY, 2011, 25 (03) : 323 - 329
[24] EFFECT OF METHOD OF SEEDING AND LEVEL OF NITROGEN ON YIELD AND YIELD ATTRIBUTES OF RICE (ORYZA-SATIVA) UNDER FLOOD-AFFECTED CONDITION
SINGH, G
SINGH, OP
INDIAN JOURNAL OF AGRONOMY, 1993, 38 (04) : 551 - 554
[25] Investigation of tissue level distribution of functional groups and associated trace metals in rice seeds (Oryza sativa L.) using FTIR and LA-ICP-MS
Basnet, Priyanka
Amarasiriwardena, Dulasiri
Wu, Fengchang
Fu, Zhiyou
Zhang, Tao
MICROCHEMICAL JOURNAL, 2016, 127 : 152 - 159
[26] An efficient and reproducible method for regeneration of whole plants from mature seeds of a high yielding Indica rice (Oryza sativa L.) variety PAU 201
Wani, Shabir H.
Sanghera, Gulzar S.
Gosal, Satbir S.
NEW BIOTECHNOLOGY, 2011, 28 (04) : 418 - 422
[27] Assessment of host plant resistance in Oryza sativa to the African rice gall midge, Orseolia oryzivora Harris and Gagne (Dipt., Cecidomyiidae), with a description of a new method for screening under artificial infestation
Williams, CT
Ukwungwu, MN
Singh, BN
Okhidievbie, O
JOURNAL OF APPLIED ENTOMOLOGY-ZEITSCHRIFT FUR ANGEWANDTE ENTOMOLOGIE, 2001, 125 (06): : 341 - 349
[28] A simple method for extracting both active oily and water soluble extract (WSE) from Nigella sativa (L.) seeds using a single solvent system
Babar, Z. M.
Azizi, Wan Mohd
Ichwan, Solachuddin J. A.
Ahmed, Qamar Uddin
Azad, Abul Kalam
Mawa, Imranul
NATURAL PRODUCT RESEARCH, 2019, 33 (15) : 2266 - 2270
[29] Evaluation of promising upland rice (Oryza sativa) varieties and their response to method of stand establishment, weed management and nutrient level under phosphorus-deficient soils of Orissa
Singh, YV
Behera, UK
Jha, KP
INDIAN JOURNAL OF AGRICULTURAL SCIENCES, 1996, 66 (12): : 718 - 721
[30] Determination of aflatoxin B1 level in rice (Oryza sativa L.) through near-infrared spectroscopy and an improved simulated annealing variable selection method
Ong, Pauline
Tung, I-Chun
Chiu, Ching-Feng
Tsai, I-Lin
Shih, Hsi-Chang
Chen, Suming
Chuang, Yung-Kun
FOOD CONTROL, 2022, 136

← 1 2 3 →