A simple method for determining Aphelenchoides besseyi infestation level of Oryza sativa seeds

被引：0
|
作者：
Hoshino, S [1 ]
Togashi, K
机构：
[1] Hiroshima Univ, Fac Integrated Arts & Sci, Higashihiroshima, Hiroshima 7398521, Japan
[2] Hiroshima Prefectural Agr Res Ctr, Higashihiroshima, Hiroshima 7390151, Japan
来源：
JOURNAL OF NEMATOLOGY | 1999年 / 31卷 / 04期
关键词：
Aphelenchoides besseyi; assay; extraction; methodology; nematode; nematode load; Oryza sativa; rice; white tip disease;
D O I：
暂无
中图分类号：
Q95 [动物学];
学科分类号：
071002 ;
摘要：
A simple extraction method was developed for obtaining the white tip nematode, Aphelenchoides besseyi Christie, from single seeds of rice, Oryza sativa. The method was as follows: Individual rice seeds were split longitudinally and then transferred into single pipet tips. Tips containing a split seed were then singly placed upright in glass vials dth water to extract the nematodes. This method was more efficient than the Baermann funnel technique and allowed nearly 100% recovery of living A. besseyi from single rice seeds within 4 hours.
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页码：641 / 643
页数：3
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