Branched peptide actuators for enzyme responsive hydrogel particles

被引:30
|
作者
McDonald, Tom O. [1 ,3 ]
Qu, Honglei [1 ,2 ,3 ]
Saunders, Brian R. [1 ]
Ulijn, Rein V. [1 ,2 ,3 ]
机构
[1] Univ Manchester, Manchester Mat Sci Ctr, Manchester M1 7HS, Lancs, England
[2] WestCHEM Dept Pure & Appl Chem, Glasgow G1 1XL, Lanark, Scotland
[3] Univ Manchester, Manchester Interdisciplinary Bioctr, Manchester M1 7DN, Lancs, England
基金
英国生物技术与生命科学研究理事会; 英国工程与自然科学研究理事会;
关键词
PROTEASES; RELEASE; SYSTEMS;
D O I
10.1039/b818174h
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
We demonstrate the preparation of enzyme responsive poly(ethylene glycol) acrylamide hydrogel microparticles (mu PEGA) functionalised by solid phase synthesis with new branched peptide actuators. Branched peptide actuators provide enhanced charge density and overcome electrostatic screening at physiological ionic strength when compared to linear ones which do not show triggered swelling under these conditions. Particle swelling was induced by enzymatic hydrolysis which caused a change in the charge balance of the branched peptide actuators from zwitterionic (neutral) to cationic. Analysis of enzymatic activity and accessibility was undertaken using fluorescence labelling and two-photon microscopy. These experiments revealed that thermolysin could access the core of particles when linear peptides are used, while access was restricted to the surface when using branched actuators. These responsive mPEGA particles were then loaded with a fluorescent labeled dextran by application of a sequential pH change. The payload could be selectively released at physiological ionic strength when exposed to the target enzyme.
引用
收藏
页码:1728 / 1734
页数:7
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