Differentiation of 'Candidatus Phytoplasma cynodontis' Based on 16S rRNA and groEL Genes and Identification of a New Subgroup, 16SrXIV-C

被引:15
|
作者
Mitrovic, J. [1 ]
Smiljkovic, M. [1 ]
Seemueller, Erich [2 ]
Reinhardt, Richard
Huettel, Bruno [3 ]
Buettner, Carmen [4 ]
Bertaccini, Assunta [5 ]
Kube, Michael [4 ]
Duduk, Bojan [1 ]
机构
[1] Inst Pesticides & Environm Protect, Lab Appl Phytopathol, Belgrade, Serbia
[2] Julius Kuehn Inst, Fed Res Ctr Cultivated Plants, Inst Plant Protect Fruit Crops & Viticulture, Dossenheim, Germany
[3] Max Planck Genome Ctr Cologne, Cologne, Germany
[4] Humboldt Univ, Dept Crop & Anim Sci, D-10099 Berlin, Germany
[5] Univ Bologna, DipSA, Plant Pathol, Alma Mater Studiorum, Bologna, Italy
关键词
WHITE LEAF DISEASE; MOLECULAR CHARACTERIZATION; FINER DIFFERENTIATION; CLASSIFICATION; YELLOWS; SEQUENCE; STRAINS;
D O I
10.1094/PDIS-01-15-0061-RE
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
'Candidatus Phytoplasma cynodontis' is widespread in bermudagrass and has only been found in monocotyledonous plants. Molecular studies carried out on strains collected in Italy, Serbia, and Albania enabled verification of molecular variability in the 16S ribosomal RNA (rRNA) gene. Based on restriction fragment length polymorphism and sequence analyses, the strains from Serbia were clearly differentiated from all others and assigned to a new ribosomal DNA (rDNA) subgroup designated as 16SrXIV-C. A system for amplification of fragments containing the 'Ca. P. cynodontis' groEL gene was developed to enable study of its variability in related strains belonging to different 16SrXIV subgroups. Despite the fact that the groEL gene exhibited a greater sequence variation than 16S rRNA, the phylogenetic tree based on groEL gene sequence analysis was highly congruent with the 16S rDNA-based tree. The groEL gene analyses supported differentiation of the Serbian strains and definition of the new subgroup 16SrXIV-C. Phylogenetic analyses of both genes confirmed distinct phylogenetic lineages for strains belonging to 16SrXIV subgroups. Furthermore, groEL is the only nonribosomal marker developed for characterization of 'Ca. P. cynodontis' thus far, and its application in molecular surveys should provide better insight into the relationships among these phytoplasmas and correlation between strain differentiation and their geographical distribution.
引用
收藏
页码:1578 / 1583
页数:6
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