Testosterone determination using rapid heterogeneous competitive-binding for enzyme-linked immunosorbent assay in flow injection.

被引：0

作者：

DiBenedetto, LT ^{[1
]}

Dimitrakopoulos, T ^{[1
]}

Davy, RM ^{[1
]}

Iles, PJ ^{[1
]}

机构：

[1] ROYAL MELBOURNE INST TECHNOL, DEPT APPL SCI, BUNDOORA, VIC, AUSTRALIA

来源：

ANALYTICAL LETTERS | 1996年 / 29卷 / 12期

关键词：

testosterone; enzyme-linked immunosorbent assay; ELISA protocol; incubation time; flow injection;

D O I：

暂无

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of a rapid competitive enzyme-linked immunosorbent assay (ELISA) for the measurement of testosterone in flow injection (FI) is presented. The ELISA protocol for the determination of testosterone was performed in 60 min compared to the standard protocol time of 150 min. This was achieved by reducing the antibody incubation time. The ELISA was used successfully to determine testosterone levels in clinical samples of both male and female subjects, and were in good agreement compared to a pathology laboratory which employed an immunoradiometric assay (IRMA).

引用

页码：2125 / 2139

页数：15

共 50 条

[21] A flow-based enzyme-linked immunosorbent assay on a polydimethylsiloxane microchip for the rapid determination of immunoglobulin A
Nakajima, Hizuru
Yagi, Maiko
Kudo, Yuki
Nakagama, Tatsuro
Shimosaka, Takuya
Uchiyama, Katsurr
TALANTA, 2006, 70 (01) : 122 - 127
[22] Methodology for determination of plasma cortisol in fish using competitive enzyme-linked immunosorbent assay (ELISA)
Velasco-Santamaria, Yohana
Cruz-Casallas, Pablo
REVISTA MVZ CORDOBA, 2007, 12 (01) : 869 - 877
[23] AN ENZYME-LINKED IMMUNOSORBENT-ASSAY (ELISA) FOR PLASMA TESTOSTERONE
ELDER, PA
LEWIS, JG
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 1985, 22 (05): : 635 - 638
[24] Testosterone Concentration in Human Tear Film Using Enzyme-linked Immunosorbent Assay
Puangmalai, Nutchaarpa
Pan, Hsuan-Yeh
Shyam, Raji
Tichenor, Anna A.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 2024, 65 (07)
[25] Development of a rapid enzyme-linked immunosorbent assay (ELISA) for gliadin determination in food
Ayob, M. K.
Rittenburg, J.
Allen, J. C.
Smith, C. J.
FOOD HYDROCOLLOIDS, 1988, 2 (01) : 39 - 49
[26] Simple, rapid enzyme-linked immunosorbent assay (ELISA) for the determination of rat osteocalcin
Fu, JY
Muller, D
CALCIFIED TISSUE INTERNATIONAL, 1999, 64 (03) : 229 - 233
[27] Simple, Rapid Enzyme-Linked Immunosorbent Assay (ELISA) for the Determination of Rat Osteocalcin
J. Y. Fu
D. Muller
Calcified Tissue International, 1999, 64 : 229 - 233
[28] Direct competitive enzyme-linked immunosorbent assay for saxitoxin and neosaxitoxin
Huang, X
Hsu, KH
Chu, FS
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1996, 44 (04) : 1029 - 1035
[29] HOMOGENEOUS ENZYME-LINKED COMPETITIVE-BINDING ASSAY FOR BIOTIN BASED ON THE AVIDIN-BIOTIN INTERACTION
DAUNERT, S
BACHAS, LG
MEYERHOFF, ME
ANALYTICA CHIMICA ACTA, 1988, 208 (1-2) : 43 - 52
[30] Direct Competitive Enzyme-linked Immunosorbent Assay for Fenvalerate Analysis
You Hai-Qin
Liu Lang
Liu Shu-Zhao
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY, 2009, 37 (04) : 577 - 580

← 1 2 3 4 5 →