Testosterone determination using rapid heterogeneous competitive-binding for enzyme-linked immunosorbent assay in flow injection.

被引：0
|
作者：
DiBenedetto, LT [1 ]
Dimitrakopoulos, T [1 ]
Davy, RM [1 ]
Iles, PJ [1 ]
机构：
[1] ROYAL MELBOURNE INST TECHNOL, DEPT APPL SCI, BUNDOORA, VIC, AUSTRALIA
来源：
ANALYTICAL LETTERS | 1996年 / 29卷 / 12期
关键词：
testosterone; enzyme-linked immunosorbent assay; ELISA protocol; incubation time; flow injection;
D O I：
暂无
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
The application of a rapid competitive enzyme-linked immunosorbent assay (ELISA) for the measurement of testosterone in flow injection (FI) is presented. The ELISA protocol for the determination of testosterone was performed in 60 min compared to the standard protocol time of 150 min. This was achieved by reducing the antibody incubation time. The ELISA was used successfully to determine testosterone levels in clinical samples of both male and female subjects, and were in good agreement compared to a pathology laboratory which employed an immunoradiometric assay (IRMA).
引用
收藏
页码：2125 / 2139
页数：15
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