A comparison of pro-inflammatory cytokine mRNA expression in equine bronchoalveolar lavage (BAL) and peripheral blood

被引：12
|
作者：
Hansen, S. [1 ]
Baptiste, K. E. [1 ]
Fjeldborg, J. [1 ]
Betancourt, A. [2 ]
Horohov, D. W. [2 ]
机构：
[1] Univ Copenhagen, Fac Hlth & Med Sci, Dept Large Anim Sci, DK-2630 Taastrup, Denmark
[2] Univ Kentucky, Dept Vet Sci, Maxwell H Gluck Equine Res Ctr, Lexington, KY 40546 USA
来源：
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2014年 / 158卷 / 3-4期
关键词：
BAL; Equine; Aging; Polymerase chain reaction; Pro-inflammatory cytokines; RECURRENT AIRWAY-OBSTRUCTION; AGE-RELATED-CHANGES; GENE-EXPRESSION; IFN-GAMMA; AFFECTED HORSES; HEAVES; DISEASE; CELLS; LYMPHOCYTES; INTERLEUKIN-8;
D O I：
10.1016/j.vetimm.2014.02.001
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
An imbalance in various cytokine mRNA expression in bronchoalveolar lavage (BAL) and peripheral blood mononuclear cells (PBMC) in horses suffering from recurrent airway obstruction (RAO) has been demonstrated; however, the natural variation of cytokine expression in healthy horses has yet to be described. The objectives of this study were to: (1) identify age-related differences in the expression of pro-inflammatory cytokines in BAL and PBMC, (2) compare the expression of proinflammatory cytokines between BAL and PBMC, and (3) evaluate the relationship between BAL pro-inflammatory cytokine expression and BAL cytology. Cells from BAL and PBMC were collected from 66 horses and stimulated with LPS and PGN. The expression of IL-1I3, IL-6, IL-8, TNF-ce and TL-4 were measured using OCR. A significant age-related decrease for IL-113 and IL-8 in BAL, significant difference in cytokine mRNA expressions between BAL and PBMC and a significant relationship between TLR-4 and IL-8 in LPS stimulated BAL samples and the percentage of neutrophils in BAL cytology were found. In conclusion, the natural age-related decrease in pro-inflammatory cytokine expression in BAL is new knowledge; this in contrast to other age-related findings associated with PBMC, where pro-inflammatory cytokines are known to increase with age in both horses and humans, the phenomenon known as `inflamm-aging'. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：238 / 243
页数：6
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