Intracellular localization of Equine herpesvirus type 1 tegument protein VP22

被引：8
|
作者：
Okada, Ayaka [1 ]
Kodaira, Akari [1 ]
Hanyu, Sachiko [1 ]
Izume, Satoko [1 ]
Ohya, Kenji [1 ,2 ]
Fukushi, Hideto [1 ,2 ]
机构：
[1] Gifu Univ, United Grad Sch Vet Sci, Dept Appl Vet Sci, Gifu 5011193, Japan
[2] Gifu Univ, Fac Appl Biol Sci, Lab Vet Microbiol, Gifu 5011193, Japan
来源：
VIRUS RESEARCH | 2014年 / 192卷
关键词：
Equine herpesvirus 1; Tegument; VP22; Localization; SIMPLEX-VIRUS; BOVINE HERPESVIRUS-1; NUCLEAR-LOCALIZATION; UL49; HOMOLOG; GENE; INFECTION; MUTANTS; ABSENCE; GROWTH; CELLS;
D O I：
10.1016/j.virusres.2014.08.006
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Intracellular localization of Equine herpesvirus type 1 (EHV-1) tegument protein VP22 was examined by using a plasmid that expressed VP22 fused with an enhanced green fluorescent protein (EGFP). Also a recombinant EHV-1 expressing VP22 fused with a red fluorescent protein (mCherry) was constructed to observe the localization of VP22 in infected cells. When EGFP-fused VP22 was overexpressed in the cells, VP22 localized in the cytoplasm and nucleus. Live cell imaging suggested that the fluorescently tagged VP22 also localized in the cytoplasm and nucleus. These results show that VP22 localizes in the cytoplasm and nucleus independently of other viral proteins. Experiments with truncation mutants of pEGFP-VP22 suggested that 154-188 aa might be the nuclear localization signal of EHV-1 VP22. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：103 / 113
页数：11
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