Multilocus Sequence Typing analysis of human Campylobacter coli in Granada (Spain)

被引：0
|
作者：
Antonio Carrillo-Avila, Jose [1 ]
Sorlozano-Puerto, Antonio [2 ]
Perez-Ruiz, Mercedes [3 ]
Gutierrez-Fernandez, Jose [2 ,3 ]
机构：
[1] Biobanco Sistema Sanitario Publ Andaluz BBSSP, Granada, Spain
[2] Univ Granada, Fac Med, Dept Microbiol, Granada, Spain
[3] Complejo Hosp Univ Granada, Microbiol Lab, Hosp Virgen de las Nieves, Granada, Spain
来源：
REVISTA ESPANOLA DE QUIMIOTERAPIA | 2016年 / 29卷 / 06期
关键词：
Campylobacter coli; typing methods; Sequence Typing; MLST; diarrhoea; BROILER FLOCKS; JEJUNI; SUBTYPES; POULTRY; STRAINS; CATTLE;
D O I：
暂无
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Introduction. Different subtypes of Campylobacter spp. have been associated with diarrhoea and a Multilocus Sequence Typing (MLST) method has been performed for subtyping. In the present work, MLST was used to analyse the genetic diversity of eight strains of Campylobacter coli. Material and methods. Nineteen genetic markers were amplified for MLST analysis: AnsB, DmsA, ggt, Cj1585c, CJJ81176-1367/1371, Tlp7, cj1321-cj1326, fucP, cj0178, cj0755/cfrA, ceuE, pldA, cstII, cstIII. After comparing the obtained sequences with the Campylobacter MLST database, the allele numbers, sequence types (STs) and clonal complexes (CCs) were assigned. Results. The 8 C. coli isolates yielded 4 different STs belonging to 2 CCs. Seven isolates belong to ST-828 clonal complex and only one isolate belong to ST-21. Two samples came from the same patient, but were isolated in two different periods of time. Conclusions. MLST can be useful for taxonomic characterization of C. coli isolates.
引用
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页码：332 / 335
页数：4
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