Interruptin B induces brown adipocyte differentiation and glucose consumption in adipose-derived stem cells

被引:10
|
作者
Kaewsuwan, Sireewan [1 ,2 ]
Plubrukarn, Anuchit [1 ]
Utsintong, Maleeruk [3 ]
Kim, Seok-Ho [4 ]
Jeong, Jin-Hyun [5 ,6 ]
Cho, Jin Gu [7 ]
Park, Sang Gyu [7 ]
Sung, Jong-Hyuk [5 ,6 ]
机构
[1] Prince Songkla Univ, Fac Pharmaceut Sci, Phytomed & Pharmaceut Biotechnol Excellence Ctr, Dept Pharmacognosy & Pharmaceut Bot, Hat Yai 90110, Thailand
[2] Prince Songkla Univ, Fac Pharmaceut Sci, Phytomed & Pharmaceut Biotechnol Excellence Ctr, Excellent Res Lab, Hat Yai 90110, Thailand
[3] Univ Phayao, Sch Pharmaceut Sci, Phayao 56000, Thailand
[4] CHA Univ, Coll Pharm, Dept Pharm, Pochon 487600, South Korea
[5] Yonsei Univ, Coll Pharm, Dept Pharmaceut, 162-1 Songdo Dong, Inchon 405750, South Korea
[6] Yonsei Univ, Coll Pharm, Inst Pharmaceut Sci, 162-1 Songdo Dong, Inchon 405750, South Korea
[7] Ajou Univ, Coll Pharm, Dept Pharm, 206 Worldcup Ro, Suwon 16499, South Korea
关键词
interruptin B; Cyclosorus terminans; adipose-derived stem cell; brown adipocyte; peroxisome proliferator-activated receptors; glucose uptake; MYOCARDIAL-INFARCTION; PPAR-GAMMA; OBESITY; TISSUE; ROSIGLITAZONE; ORLISTAT; RISK;
D O I
10.3892/mmr.2016.4758
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Interruptin B has been isolated from Cyclosorus terminans, however, its pharamcological effect has not been fully identified. In the present study, the effects of interruptin B, from C. terminans, on brown adipocyte differentiation and glucose uptake in adipose-derived stem cells (ASCs) were investigated. The results revealed that interruptin B dose-dependently enhanced the adipogenic differentiation of ASCs, with an induction in the mRNA expression levels of peroxisome proliferator-activated receptor (PPAR)- and PPAR-. In addition, interruptin B efficiently increased the number and the membrane potential of mitochondria and upregulated the mRNA expression levels of uncoupling protein (UCP)-1 and cyclooxygenase (COX)-2, which are all predominantly expressed in brown adipocytes. Interruptin B increased glucose consumption in differentiated ASCs, accompanied by the upregulation in the mRNA expression levels of glucose transporter (GLUT)-1 and GLUT-4. The computational analysis of molecular docking, a luciferase reporter assay and surface plasmon resonance confirmed the marked binding affinity of interruptin B to PPAR- and PPAR- (K-D values of 5.32 and 0.10 mu M, respectively). To the best of our knowledge, the present study is the first report to show the stimulatory effects of interruptin B on brown adipocyte differentiation and glucose uptake in ASCs, through its role as a dual PPAR- and PPAR- ligand. Therefore, interruptin B could be further developed as a therapeutic agent for the treatment of diabetes.
引用
收藏
页码:2078 / 2086
页数:9
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