Production of polyclonal antiserum specific to the 27.5 kDa envelope protein of white spot syndrome virus

被引：20
|
作者：
You, ZO [1 ]
Nadala, ECB
Yang, JS
van Hulten, MCW
Loh, PC
机构：
[1] Univ Hawaii, Dept Microbiol, Honolulu, HI 96822 USA
[2] Univ Cambridge, Dept Haematol, Cambridge CB2 2PT, England
[3] Univ Wageningen & Res Ctr, Virol Lab, NL-6709 PD Wageningen, Netherlands
来源：
DISEASES OF AQUATIC ORGANISMS | 2002年 / 51卷 / 01期
关键词：
white spot syndrome virus; 27.5 kDa structural protein; rabbit polyclonal antiserum;
D O I：
10.3354/dao051077
中图分类号：
S9 [水产、渔业];
学科分类号：
0908 ;
摘要：
A truncated version of the white spot syndrome virus (WSSV) 27.5 kDa envelope protein was expressed as a histidine tag fusion protein in Escherichia coli. The bacterial expression system allowed the production of up to 10 mg of purified recombinant protein per liter of bacterial culture. Antiserum from a rabbit immunized with the recombinant protein recognized the 27.5 kDa viral envelope protein of WSSV isolated from different geographic regions. The antiserum did not recognize any of the other known WSSV structural proteins. A sensitive immunodot assay for WSSV was developed using the specific rabbit polyclonal antiserum.
引用
收藏
页码：77 / 80
页数：4
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