The downregulation of microRNA-375 in human cervical squamous cell carcinoma promotes invasion and migration by targeting JAK2

Micro RNAs (miRNAs) contribute to the progression and expansion of many cancers, including cervical cancer. Reports reveal the down-regulation of MicroRNA-375 (miR-375) within cervical cancer tissues. Nevertheless, the mechanism for regulation of cervical cancer progression by miR-375 is still unknown. The current work deals with the molecular mechanisms orienting the regulatory impacts of miR-375 on invasion and migration in human cervical carcinoma. miRNA expression within cervical cancer tissues was assessed through Stem-loop RT-PCR. The invasion and migration of miR-375 mimic-transfected SiHa cells were observed by the Transwell test and wound healing test, respectively. Bioinformatics analysis was performed for predicting the target gene of miR-375, which was validated by the dual-luciferase test. Real-time RT-PCR and Western blot analysis were utilized for detecting protein expression and mRNA transcription. In cervical cancer, miR-375 expression was 4.2 +/- 0.15-fold lower compared to the adjacent noncancerous tissues. The number of invasive and migrated SiHa cells (32 +/- 3.6; 21.86 +/- 6.4) transfected with miR-375 mimics were considerably lower in comparison with the NC group (101 +/- 7.5; 41 +/- 5.6) after 24 hours. Real-time RT-PCR and Western blot analysis confirmed JAK2 as a downstream target gene of miR-375. The protein expression and mRNA level of JAK2 in miR-375 mimic-transfected SiHa cells were decreased. The present study indicated that the invasion and migration of SiHa cells are regulated by miR-375 through down regulating JAK2 expression. This study provides a theoretical and experimental basis for reversing the metastasis of cervical cancer and developing new candidate drugs.