Studies of unicellular microorganisms Saccharomyces cerevisiae by means of positron annihilation lifetime spectroscopy

被引：19

作者：

Kubicz, Ewelina ^{[1
]}

Jasinska, Bozena ^{[3
]}

Zgardzinska, Bozena ^{[3
]}

Bednarski, Tomasz ^{[1
]}

Bialas, Piotr ^{[1
]}

Czerwinski, Eryk ^{[1
]}

Gajos, Aleksander ^{[1
]}

Gorgol, Marek ^{[3
]}

Kaminska, Daria ^{[1
]}

Kaplon, Lukasz ^{[1
,2
]}

Kochanowski, Andrzej ^{[4
]}

Korcyl, Grzegorz ^{[1
]}

Kowalski, Pawel ^{[5
]}

Kozik, Tomasz ^{[1
]}

Krzemien, Wojciech ^{[6
]}

Niedzwiecki, Szymon ^{[1
]}

Palka, Marek ^{[1
]}

Raczynski, Lech ^{[5
]}

Rajfur, Zenon ^{[1
]}

Rudy, Zbigniew ^{[1
]}

Rundel, Oleksandr ^{[1
]}

Sharma, Neha Gupta ^{[1
]}

Silarski, Michal ^{[1
]}

Slomski, Artur ^{[1
]}

Strzelecki, Adam ^{[1
]}

Wieczorek, Anna ^{[1
,2
]}

Wislicki, Wojciech ^{[5
]}

Zielinski, Marcin ^{[1
]}

Moskal, Pawel ^{[1
]}

机构：

[1] Jagiellonian Univ, Fac Phys Astron & Appl Comp Sci, PL-30348 Krakow, Poland

[2] PAS, Inst Met & Mat Sci, PL-30059 Krakow, Poland

[3] Marie Curie Sklodowska Univ, Inst Phys, Dept Nucl Methods, PL-20031 Lublin, Poland

[4] Jagiellonian Univ, Fac Chem, Dept Chem Technol, PL-30059 Krakow, Poland

[5] Natl Ctr Nucl Res, Swierk Comp Ctr, PL-05400 Otwock, Poland

[6] Natl Ctr Nucl Res, Div High Energy Phys, PL-05400 Otwock, Poland

来源：

NUKLEONIKA | 2015年 / 60卷 / 04期

关键词：

environmental scanning electron microscopy (ESEM); free volume; inverted microscopy; positron annihilation; positronium; yeasts; HIT-POSITION RECONSTRUCTION; PLASTIC SCINTILLATORS; LIBRARY; SIGNALS; TIME;

D O I：

10.1515/nuka-2015-0135

中图分类号：

O61 [无机化学];

学科分类号：

070301 ; 081704 ;

摘要：

Results of positron annihilation lifetime spectroscopy (PALS) and microscopic studies on simple microorganisms, brewing yeasts, are presented. Lifetime of ortho-positronium (o-Ps) were found to change from 2.4 to 2.9 ns (longer-lived component) for lyophilized and aqueous yeasts, respectively. Also hygroscopicity of yeasts in time was examined, allowing to check how water - the main component of the cell - affects PALS parameters, thus lifetime of o-Ps were found to change from 1.2 to 1.4 ns (shorter-lived component) for the dried yeasts. The time sufficient to hydrate the cells was found below 10 hours. In the presence of liquid water, an indication of reorganization of yeast in the molecular scale was observed. Microscopic images of the lyophilized, dried, and wet yeasts with best possible resolution were obtained using inverted microscopy (IM) and environmental scanning electron microscopy (ESEM) methods. As a result, visible changes to the surface of the cell membrane were observed in ESEM images.

引用

页码：749 / 753

页数：5

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