Internal Electrostatic Control of the Primary Charge Separation and Recombination in Reaction Centers from Rhodobacter sphaeroides Revealed by Femtosecond Transient Absorption

被引：19

作者：

Gibasiewicz, K. ^{[1
]}

Pajzderska, M. ^{[1
]}

Ziolek, M. ^{[1
]}

Karolczak, J. ^{[1
]}

Dobek, A. ^{[1
]}

机构：

[1] Adam Mickiewicz Univ Poznan, Dept Phys, PL-61614 Poznan, Poland

来源：

JOURNAL OF PHYSICAL CHEMISTRY B | 2009年 / 113卷 / 31期

关键词：

PHOTOSYNTHETIC REACTION CENTERS; BACTERIAL REACTION CENTERS; ENERGY-TRANSFER PATHWAYS; PAIR DECAY KINETICS; RHODOPSEUDOMONAS-VIRIDIS; NANOSECOND FLUORESCENCE; PRIMARY ACCEPTOR; RADICAL PAIRS; RATE-CONSTANT; BACTERIOCHLOROPHYLL;

D O I：

10.1021/jp811234q

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

We report the observation of two conformational states of closed RCs from Rhodobacter sphaeroides characterized by different P+HA- -> PHA charge recombination lifetimes, one of which is of subanosecond value (700 +/- 200 ps). These states are also characterized by different primary charge separation lifetimes. It is proposed that the distinct conformations are related to two protonation states either of reduced secondary electron acceptor, Q(A)(-), or of a titratable amino acid residue localized near Q(A). The reaction centers in the protonated state are characterized by faster charge separation and slower charge recombination when compared to those in the unprotonated state. Both effects are explained in terms of the model assuming modulation of the free energy level of the state P+HA- by the charges oil or near Q(A) and decay of the P+HA- state via the thermally activated P+BA- state.

引用

页码：11023 / 11031

页数：9

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