IDENTIFICATION OF INDIAN MANGROVE MUD CRAB GENUS SCYLLA SPP. (BRACHYURA: PORTUNIDAE) USING RAPD AND PCR-RFLP MARKERS

被引：4

作者：

Mandal, Anup ^{[1
]}

Varkey, Mathews ^{[1
]}

Mani, Anjali Kottayil ^{[1
]}

Sobhanan, Sobha Pindaniyil ^{[1
]}

Thampi-Samraj, Yohannan Chellamma ^{[1
]}

机构：

[1] Rajiv Gandhi Ctr Aquaculture, Nagapattinam 609109, Tamil Nadu, India

来源：

JOURNAL OF SHELLFISH RESEARCH | 2014年 / 33卷 / 02期

关键词：

Scylla spp; molecular identification; randomly amplified polymorphic DNA; restriction fragment length polymorphism; MITOCHONDRIAL-DNA; HAAN; AMPLIFICATION; PRIMERS;

D O I：

10.2983/035.033.0219

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Identification of commonly available Indian mud crabs of the genus Scylla was carried out using RAPD and PCR-RFLP to resolve the taxonomic ambiguity of the genus. Samples were collected from western, eastern, and Andaman coastal waters of India. A total of 16 potential species-specific RAPD markers were identified from 179 individuals using 4 arbitrary primers (OPA2, OPA14, UBC122, and UBC456). Three PCR-RFLP markers were developed that could be used to confirm taxonomic status of Indian mud crab species. The species-specific RAPD markers and the digestion pattern of PCR-RFLP analysis indicate conclusively that only 2 species of mud crabs (Scylla serrata and Scylla olivacea) are commonly present in Indian coastal waters.

引用

页码：503 / 510

页数：8

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