Cyclometalated Platinum(II) Complexes as Highly Sensitive Luminescent Switch-On Probes for Practical Application in Protein Staining and Cell Imaging

被引：136
|
作者：
Wu, Peng [1 ,2 ]
Wong, Ella Lai-Ming [1 ,2 ]
Ma, Dik-Lung [1 ,2 ]
Tong, Glenna So-Ming [1 ,2 ]
Ng, Kwan-Ming [1 ,2 ]
Che, Chi-Ming [1 ,2 ]
机构：
[1] Univ Hong Kong, Dept Chem, Inst Mol Technol Drug Discovery & Synth, Hong Kong, Hong Kong, Peoples R China
[2] Univ Hong Kong, Open Lab Chem Biol, Inst Mol Technol Drug Discovery & Synth, Hong Kong, Hong Kong, Peoples R China
来源：
CHEMISTRY-A EUROPEAN JOURNAL | 2009年 / 15卷 / 15期
关键词：
cell imaging; gels; luminescence; noncovalent interactions; platinum; protein staining; SODIUM DODECYL-SULFATE; MOLECULAR LIGHT SWITCH; POLYACRYLAMIDE GELS; DNA; BINDING; NITROCELLULOSE; SENSOR; PHOTOCHEMISTRY; LIGANDS; DEVICES;
D O I：
10.1002/chem.200802707
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A method to prepare highly luminescent cyclometalated platinum(II) complexes as switch-on probes for application in protein staining and cell imaging, was reported. The finished products showed several properties like MLCT emission in the visible spectral region, solubility and stability in aqueous staining solutions at room temperature, and binding to proteins through non-covalent interaction. The UV-visible absorption and NMR spectroscopy used for the study revealed that platinum(II) complexes remained stable in aqueous solution for 72 hours at room temperature. The cyclometalated platinum(II) complexes acted as protein-binding probes that can be used for the staining of protein mixtures in 1D and 2D SDS-PAGE without interfering with peptide-mass fingerprinting analysis and for live cell imaging. The MLCT emission of platinum(II) complexes were sensitive to auxiliary ligands.
引用
收藏
页码：3652 / 3656
页数：5
相关论文
共 23 条
  • [21] Highly selective and sensitive detection of Zn(II) and Cu(II) ions using a novel peptide fluorescent probe by two different mechanisms and its application in live cell imaging
    Wang, Peng
    Wu, Jiang
    SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY, 2019, 208 : 140 - 149
  • [22] A highly selective pyridoxal-based chemosensor for the detection of Zn(ii) and application in live-cell imaging; X-ray crystallography of pyridoxal-TRIS Schiff-base Zn(ii) and Cu(ii) complexes
    Hussain, Anwar
    Mariappan, Kadarkaraisamy
    Cork, Dawson C.
    Lewandowski, Luke D.
    Shrestha, Prem K.
    Giri, Samiksha
    Wang, Xuejun
    Sykes, Andrew G.
    RSC ADVANCES, 2021, 11 (54) : 34181 - 34192
  • [23] Induced self-assembly and disassembly of water-soluble alkynylplatinum(II) terpyridyl complexes with "switchable" near-infrared (NIR) emission modulated by metal-metal interactions over physiological pH: demonstration of pH-responsive NIR luminescent probes in cell-imaging studies
    Chung, Clive Yik-Sham
    Li, Steve Po-Yam
    Louie, Man-Wai
    Lo, Kenneth Kam-Wing
    Yam, Vivian Wing-Wah
    CHEMICAL SCIENCE, 2013, 4 (06) : 2453 - 2462
123