Fluorescent reaction between ascorbic acid and DAN and its analytical application

被引：23
|
作者：
Yang, JH [1 ]
Tong, CL [1 ]
Jie, NQ [1 ]
Zhang, GL [1 ]
Ren, XZ [1 ]
Hu, JT [1 ]
机构：
[1] HANGZHOU EVERLONG BIOTECH,HANGZHOU,PEOPLES R CHINA
来源：
TALANTA | 1997年 / 44卷 / 05期
关键词：
ascorbic acid determination; fluorescent reaction;
D O I：
10.1016/S0039-9140(96)02129-7
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Fluorescent reaction between ascorbic acid (AA) and 2,3-diamino-naphthalene (DAN) was studied. The experimental results showed that AA could react with DAN at pH = 10.2-10.5, and form the fluorescent heterocyclic condensation products which emitted strong fluorescence. The fluorescence intensity was measured in a 1 cm quartz cell with excitation and emission wavelengths of 400 and 520 nm, respectively. The relationship was obtained between the fluorescence intensity and AA concentration in the range of 2-30/mu g ml(-1), the regression coefficient is 0.9993. The detection limit (signal-to-noise = 2) is 0.4 mu g ml(-1). (C) 1997 Elsevier Science B.V.
引用
收藏
页码：855 / 858
页数：4
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