Fluorescent reaction between ascorbic acid and DAN and its analytical application

被引：23

作者：

Yang, JH ^{[1
]}

Tong, CL ^{[1
]}

Jie, NQ ^{[1
]}

Zhang, GL ^{[1
]}

Ren, XZ ^{[1
]}

Hu, JT ^{[1
]}

机构：

[1] HANGZHOU EVERLONG BIOTECH,HANGZHOU,PEOPLES R CHINA

来源：

TALANTA | 1997年 / 44卷 / 05期

关键词：

ascorbic acid determination; fluorescent reaction;

D O I：

10.1016/S0039-9140(96)02129-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescent reaction between ascorbic acid (AA) and 2,3-diamino-naphthalene (DAN) was studied. The experimental results showed that AA could react with DAN at pH = 10.2-10.5, and form the fluorescent heterocyclic condensation products which emitted strong fluorescence. The fluorescence intensity was measured in a 1 cm quartz cell with excitation and emission wavelengths of 400 and 520 nm, respectively. The relationship was obtained between the fluorescence intensity and AA concentration in the range of 2-30/mu g ml(-1), the regression coefficient is 0.9993. The detection limit (signal-to-noise = 2) is 0.4 mu g ml(-1). (C) 1997 Elsevier Science B.V.

引用

页码：855 / 858

页数：4

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