Molecular Survey on Detection of Leishmania Infection in Rodent Reservoirs in Jahrom District, Southern Iran

被引:0
|
作者
Davami, Mohammad Hassan [1 ]
Motazedian, Mohammad Hossein [2 ]
Kalantari, Mohsen [3 ]
Asgari, Qasem [2 ]
Mohammadpour, Iraj [2 ]
Sotoodeh-Jahromi, Abdolreza [1 ]
Solhjoo, Kavous [1 ]
Pourahmad, Morteza [4 ]
机构
[1] Jahrom Univ Med Sci, Sch Med, Dept Med Microbiol, Jahrom, Iran
[2] Shiraz Univ Med Sci, Dept Parasitol & Mycol, Shiraz, Iran
[3] Shiraz Univ Med Sci, Mamasani Paramed Sch, Dept Publ Hlth, Shiraz, Iran
[4] Jahrom Univ Med Sci, Dept Internal Med, Jahrom, Iran
关键词
Rodent; Leishmania; PCR; Sequencing; Iran; ZOONOTIC CUTANEOUS LEISHMANIASIS; PCR-BASED DETECTION; MERIONES-LIBYCUS; PHLEBOTOMUS-PAPATASI; MAIN RESERVOIR; TATERA-INDICA; 1ST DETECTION; NESTED-PCR; FOCUS; DIAGNOSIS;
D O I
暂无
中图分类号
R1 [预防医学、卫生学];
学科分类号
1004 ; 120402 ;
摘要
Background: Zoonotic Cutaneous Leishmaniasis (ZCL) is endemic in many parts of Iran. Recently its incidence is considerable in different parts of Jahrom district, in Fars Province, southern Iran. The aims of our study were to investigate the prevalence of leishmania infection, and identify and characterize the Leishmania species present, among the rodents by molecular methods in a new endemic focus of ZCL, in an urban and rural area of the Jahrom district, Fars Province, southern Iran. Methods: From May to November 2010), 55 rodents in four regions of Jahrom focus were caught and checked for leishmania infection by the microscopical examination of liver, spleen, ears, and footpads' smears. Results: Overall 18 Meriones persicus, 15 Tatera indica, 14 Mus musculus, and 8 Rattus rattus were caught. Totally, four (16.5%) and two (13.3%) of the Me. persicus and Ta. indica, but only one of Mu. musculus and Ra. rattus were found smear-positive for leishmania amastigotes, respectively. In the nested-PCR assay 8 (14.6%) smears were found positive for Leishmania major, none was found positive for any other Leishmania species. Sequencing based detection of Leishmania confirmed the microscopic and PCR findings. All positive specimens were shown 95-96% similarity with L. major Friedlin. Conclusion: Tatera indica and Me. persicus are incriminated as the main `reservoir' hosts of L. major in the rural area of Jahrom, moreover, Mu. musculus and Ra. rattus have the minor but remarkable role in the maintenance of the disease in the urban regions of Jahrom focus.
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页码:139 / 146
页数:8
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