A new species of Gentianella, G. tumailica sp. nov., from Tumail in the Kargil district of Ladakh, northwest Himalaya, India is described and illustrated. This novelity is morphologically similar to the sympatric G. stoliczka in inflorescence pattern, calyx and corolla shapes, sagitate anthers, more or less rounded seeds, but differs in smaller size of vegetative and floral structures, larger basal leaves, few cauline leaves, leaf apex obtuse to rounded, calyx lobes unequal (2 longer, 3 shorter), inflorescence enveloped by 4 foliaceous bracts, capsule elliptic to oblong and seed coat smooth. In addition, a diagnostic key to all Indian species of Gentianella is presented.