IS4 peptide forms ion channel in rat skeletal muscle cell membrane

被引：0
|
作者：
Bao, L
Miao, ZW
Zhou, PA [1 ]
Jiang, Y
Zhang, RJ
Tang, YQ
机构：
[1] Peking Univ, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100871, Peoples R China
[2] Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China
[3] Peking Univ, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100871, Peoples R China
来源：
CHINESE SCIENCE BULLETIN | 1999年 / 44卷 / 24期
关键词：
synthetic IS4 peptide; cation selective channel; rat skeletal myotube;
D O I：
10.1007/BF02885928
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A 22-mer peptide, identical to the primary sequence of domain I segment 4 (IS4) of rat brain sodium channel I, has been synthesized. IS4 peptide can incorporate into cultured rat skeletal myotube membranes and form ion channels. With patch clamp cell-attached technique single channel currents through IS4 channels can be recorded. The single channel conductances of IS4 channels are distributed heterogeneously. With different holding potentials, the mean open time, the mean closed time and the mean open probability are different respectively. IS4 channels are selective for Na+, Li+ and K+, but not for Cl-.
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收藏
页码：2232 / 2236
页数：5
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