Molecular cloning, sequence identification, and tissue expression profile analysis of three novel porcine genes: SDHB, SNRPA and CRYBB1

被引：5
|
作者：
An, Q. C. [1 ]
Liu, G. Y. [1 ]
机构：
[1] Yunnan Agr Univ, Key Lab Anim Nutr & Feed Yunnan Prov, Kunming 650201, Peoples R China
来源：
MOLECULAR BIOLOGY REPORTS | 2009年 / 36卷 / 04期
关键词：
Pig; SDHB; SNRPA; CRYBB1; Tissue expression analysis; SMALL NUCLEAR RIBONUCLEOPROTEIN; 70-KDA PROTEIN-COMPONENT; X-LARGE-WHITE; LONGISSIMUS-DORSI; INDUCED APOPTOSIS; RNA-BINDING; U1A PROTEIN; CLEAVAGE; PHEOCHROMOCYTOMA; CRYSTALLIN;
D O I：
10.1007/s11033-008-9229-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The complete coding sequences of three porcine genes-SDHB, SNRPA and CRYBB1 were amplified using the reverse transcriptase polymerase chain reaction (RT-PCR) based on the conserved coding sequence information of the mouse or other mammals and highly homologous pig ESTs. These three genes were then deposited into GenBank database and assigned to GeneID: 100125544, 768109 and 780429. The phylogenetic tree analysis revealed that the swine SDHB and SNRPA have closer genetic relationships with the human SDHB and SNRPA, but swine CRYBB1 has a closer genetic relationship with the bovine CRYBB1.The tissue expression analysis indicated that that swine SDHB, SNRPA and CRYBB1 gene were differentially expressed in tissues including fat, lung, muscle, small intestine, kidney, large intestine, spleen and liver. Our experiment is the first to establish the primary foundation for further research on these three swine genes.
引用
收藏
页码：683 / 690
页数：8
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