Adding new dimensions to laser-scanning fluorescence microscopy

被引：1

作者：

De, A. K. ^{[1
]}

Goswami, D. ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Kanpur 208016, Uttar Pradesh, India

来源：

JOURNAL OF MICROSCOPY | 2009年 / 233卷 / 02期

基金：

英国惠康基金;

关键词：

Confocal microscopy; fluorescence laser scanning microscope; multiphoton microscopy; second-harmonic generation; ultrafast pulsed illumination; 3-dimensional spatial resolution; 2-PHOTON; EXCITATION; PHOTON; CELLS;

D O I：

10.1111/j.1365-2818.2009.03122.x

中图分类号：

TH742 [显微镜];

学科分类号：

摘要：

We describe a novel method of optical imaging by exploiting simple ideas borrowed from pulsed optics. We show that the use of ultrafast pulsed one-photon excitation in laser-scanning fluorescence microscopy dramatically brings together several advantages offered by two widely used present day microscopic techniques, confocal and multi-photon fluorescence microscopy. The method appears as a novel tool in the context of laser-scanning fluorescence microscopy by having a 'built-in' 3D spatial resolution.

引用

页码：320 / 325

页数：6

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