Dynein-dependent transport of spindle assembly checkpoint proteins off kinetochores toward spindle poles

被引：26

作者：

Silva, Patricia M. A. ^{[1
,2
]}

Reis, Rita M. ^{[1
]}

Bolanos-Garcia, Victor M. ^{[3
]}

Florindo, Claudia ^{[2
,4
]}

Tavares, Alvaro A. ^{[2
,4
]}

Bousbaa, Hassan ^{[1
,5
,6
]}

机构：

[1] CESPU, Inst Invest & Formacao Avancada Ciencias & Tecnol, P-4585116 Gandra, PRD, Portugal

[2] Univ Algarve, CBME IBB, Ctr Mol & Struct Biomed, P-8005139 Faro, Portugal

[3] Oxford Brookes Univ, Fac Hlth & Life Sci, Dept Biol & Med Sci, Oxford OX3 0BP, England

[4] Univ Algarve, Dept Ciencias Biomed & Med, P-8005139 Faro, Portugal

[5] Univ Porto, CEQUIMED UP, Ctr Quim Med, P-4050313 Oporto, Portugal

[6] Univ Porto, CIIMAR CIMAR, Ctr Interdisciplinar Invest Marinha & Ambiental, P-4050123 Oporto, Portugal

来源：

FEBS LETTERS | 2014年 / 588卷 / 17期

关键词：

Mitotic checkpoint; Checkpoint silencing: cytoplasmic dynein; Mad1; Mad2; Bub1; BubR1; Bub3; Hec1; Mis12; MITOTIC CHECKPOINT; MICROTUBULE ATTACHMENT; CHROMOSOME ALIGNMENT; BUB3; STABILITY; LIVING CELLS; COMPLEX; APC/C; INHIBITION; CDC20; HEC1;

D O I：

10.1016/j.febslet.2014.07.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A predominant mechanism of spindle assembly checkpoint (SAC) silencing is dynein-mediated transport of certain kinetochore proteins along microtubules. There are still conflicting data as to which SAC proteins are dynein cargoes. Using two ATP reduction assays, we found that the core SAC proteins Mad1, Mad2, Bub1, BubR1, and Bub3 redistributed from attached kinetochores to spindle poles, in a dynein-dependent manner. This redistribution still occurred in metaphase-arrested cells, at a time when the SAC should be satisfied and silenced. Unexpectedly, we found that a pool of Hec1 and Mis12 also relocalizes to spindle poles, suggesting KMN components as additional dynein cargoes. The potential significance of these results for SAC silencing is discussed. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：3265 / 3273

页数：9

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