Monitoring Astrocytic Ca2+ Activity in Freely Behaving Mice

被引：16
|
作者：
Qin, Han [1 ,2 ,3 ]
He, Wenjing [1 ,2 ]
Yang, Chuanyan [4 ,5 ]
Li, Jin [1 ,2 ]
Jian, Tingliang [1 ,2 ]
Liang, Shanshan [1 ,2 ]
Chen, Tunan [4 ,5 ]
Feng, Hua [4 ,5 ]
Chen, Xiaowei [1 ,2 ]
Liao, Xiang [3 ]
Zhang, Kuan [1 ,2 ]
机构：
[1] Third Mil Med Univ, Brain Res Ctr, Chongqing, Peoples R China
[2] Third Mil Med Univ, State Key Lab Trauma Burns & Combined Injury, Chongqing, Peoples R China
[3] Chongqing Univ, Sch Med, Ctr Neurointelligence, Chongqing, Peoples R China
[4] Third Mil Med Univ, Southwest Hosp, Dept Neurosurg, Chongqing, Peoples R China
[5] Third Mil Med Univ, Southwest Hosp, Key Lab Neurotrauma, Chongqing, Peoples R China
来源：
FRONTIERS IN CELLULAR NEUROSCIENCE | 2020年 / 14卷
基金：
中国国家自然科学基金; 国家重点研发计划;
关键词：
optic fiber; astrocytes; Ca2+ signals; genetically encoded Ca2+ indicators; freely behaving mice; cortex; hippocampus; CORTICAL ASTROCYTES; NEURONAL-ACTIVITY; SENSORY RESPONSE; AUDITORY-CORTEX; CALCIUM; CIRCUIT; HYPERACTIVITY; ACTIVATION; INITIATION; DYNAMICS;
D O I：
10.3389/fncel.2020.603095
中图分类号：
Q189 [神经科学];
学科分类号：
071006 ;
摘要：
Monitoring astrocytic Ca2+ activity is essential to understand the physiological and pathological roles of astrocytes in the brain. However, previous commonly used methods for studying astrocytic Ca2+ activities can be applied in only anesthetized or head-fixed animals, which significantly affects in vivo astrocytic Ca2+ dynamics. In the current study, we combined optic fiber recordings with genetically encoded Ca2+ indicators (GECIs) to monitor astrocytic activity in freely behaving mice. This approach enabled selective and reliable measurement of astrocytic Ca2+ activity, which was verified by the astrocyte-specific labeling of GECIs and few movement artifacts. Additionally, astrocytic Ca2+ activities induced by locomotion or footshock were stably recorded in the cortices and hippocampi of freely behaving mice. Furthermore, this method allowed for the longitudinal study of astrocytic activities over several weeks. This work provides a powerful approach to record astrocytic activity selectively, stably, and chronically in freely behaving mice.
引用
收藏
页数：11
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