Functional PLGA Scaffolds for Chondrogenesis of Bone-Marrow-Derived Mesenchymal Stem Cells

被引：21
|
作者：
Park, Kwideok [1 ]
Cho, Kyoung-Jin [1 ]
Kim, Jae-Jin [1 ]
Kim, Ik-Hwan [2 ]
Han, Dong Keun [1 ]
机构：
[1] Korea Inst Sci & Technol, Biomat Res Ctr, Seoul 130650, South Korea
[2] Korea Univ, Sch Life Sci & Biotechnol, Seoul, South Korea
来源：
MACROMOLECULAR BIOSCIENCE | 2009年 / 9卷 / 03期
关键词：
drug delivery systems; structure; supports; surfaces; tissue engineering; INDUCED GRAFT-POLYMERIZATION; GROWTH-FACTOR DELIVERY; IN-VIVO; TISSUE REGENERATION; DIFFERENTIATION; HEPARIN; TGF-BETA-1; SYSTEM; HYDROGELS; ADHESION;
D O I：
10.1002/mabi.200800187
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Two chondrogenic factors, Dex and TGF-beta 1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-beta 1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II Collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-beta 1.
引用
收藏
页码：221 / 229
页数：9
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