Functional PLGA Scaffolds for Chondrogenesis of Bone-Marrow-Derived Mesenchymal Stem Cells

被引：21

作者：

Park, Kwideok ^{[1
]}

Cho, Kyoung-Jin ^{[1
]}

Kim, Jae-Jin ^{[1
]}

Kim, Ik-Hwan ^{[2
]}

Han, Dong Keun ^{[1
]}

机构：

[1] Korea Inst Sci & Technol, Biomat Res Ctr, Seoul 130650, South Korea

[2] Korea Univ, Sch Life Sci & Biotechnol, Seoul, South Korea

来源：

MACROMOLECULAR BIOSCIENCE | 2009年 / 9卷 / 03期

关键词：

drug delivery systems; structure; supports; surfaces; tissue engineering; INDUCED GRAFT-POLYMERIZATION; GROWTH-FACTOR DELIVERY; IN-VIVO; TISSUE REGENERATION; DIFFERENTIATION; HEPARIN; TGF-BETA-1; SYSTEM; HYDROGELS; ADHESION;

D O I：

10.1002/mabi.200800187

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two chondrogenic factors, Dex and TGF-beta 1, were incorporated into PLGA scaffolds and their chondrogenic potential was evaluated. The Dex-loaded PLGA scaffold was grafted with AA and heparin, the heparin-immobilized one was then reacted with TGF-beta 1, yielding a PLGA/Dex-TGF (PLGA/D/T) scaffold. The scaffolds were seeded with rabbit MSCs and cultured for 4 weeks. The results show that the scaffolds including chondrogenic factors strongly upregulated the expression of cartilage-specific genes and clearly displayed type-II Collagen immunofluorescence. The functionalized PLGA scaffolds could provide an appropriate niche for chondrogenic differentiation of MSC without a constant medium supply of Dex and TGF-beta 1.

引用

页码：221 / 229

页数：9

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