Determination of Tirofiban in human serum by liquid chromatography-tandem mass spectrometry

被引：10
|
作者：
Oertel, R
Köhler, A
Koster, A
Kirch, W
机构：
[1] Tech Univ Dresden, Med Fac Carl Gustav Carus, Inst Clin Pharmacol, D-01307 Dresden, Germany
[2] Deutsch Herzzentrum Berlin, D-13353 Berlin, Germany
来源：
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2004年 / 805卷 / 01期
关键词：
Tirofiban;
D O I：
10.1016/j.jchromb.2004.03.003
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
A liquid chromatography-tandem mass spectrometric (LC-MS-MS) method with a rapid and simple sample preparation was developed and validated for the determination of Tirofiban in biological fluids. Tirofiban in serum samples was extracted and cleaned up by using an automated solid phase extraction method. An external calibration was used. The mass spectrometer was operated in the multiple reaction monitoring mode (MRM). A good linear response over the range of 2-200 ng/ml was demonstrated. The accuracy for Tirofiban ranged from 94.8 to 110.8% within-day and from 103.0 to 104.7% between-day. The lower limit of quantification was 2 ng/ml. This method is suitable for pharmacokinetic studies. (C) 2004 Elsevier B.V. All rights reserved.
引用
收藏
页码：181 / 185
页数：5
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